FB2024_03 , released June 25, 2024
Allele: Dmel\Sod2tetO.cCa
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General Information
Symbol
Dmel\Sod2tetO.cCa
Species
D. melanogaster
Name
Tn10 tetracycline operator construct a of Curtis
FlyBase ID
FBal0246446
Feature type
allele
Associated gene
Dmel\Sod2
Associated Insertion(s)
Carried in Construct
P{DOX-Sod2.C}
Key Links
Transgenic product class
cDNA
(Curtis et al., 2007)
wild_type
(Curtis et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Curtis et al., 2007)
wild_type
(Curtis et al., 2007)
Mutagen
in vitro construct
(Curtis et al., 2007)
Progenitor genotype
Carried in construct
P{DOX-Sod2.C}
Cytology
Description

tetO sequences regulate expression of a Sod2 cDNA.

(Curtis et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
tetO
Encoded product / tool
Sod2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Doxycycline (DOX)-induced overexpression of Sod2Tn10\tetO.cCa driven by Tn10\tetRAct5C.T:Hsim\VP16 in adults results in increased lifespan compared with controls, while larval overexpression has no detectable effect on subsequent adult lifespan. Plots of log mortality rate versus age reveal that the increase in lifespan is due primarily to a rapid decrease in the initial mortality rate (within approximately 48 hours of DOX feeding), with no detectable effect on the mortality rate doubling time.

      Sod2Tn10\tetO.cCa overexpression yields no increase in resistance to the stressors hydrogen peroxide, paraquat, 100% oxygen atmosphere, or desiccation compared with controls.

      Sod2Tn10\tetO.cCa overexpressing flies exhibit normal even increased total lifetime locomotor activity compared with controls.

      Doxycycline (DOX)-induced overexpression of Sod2Tn10\tetO.cCa driven by Tn10\tetRAct5C.T:Hsim\VP16 results in diminished thermotolerance with reductions in mean life span compared with controls.

      Doxycycline (DOX)-induced overexpression of Sod2Tn10\tetO.cCa driven by Tn10\tetRAct5C.T:Hsim\VP16 results in a significant decrease in CO[[2]] production compared with controls.

      (Curtis et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sod2Tn10\tetO.cCa
      Sod2tetO.cCa
      Name Synonyms
      Tn10 tetracycline operator construct a of Curtis
      Secondary FlyBase IDs
        References (1)