FlyBase Allele Report: Dmel\Cip4[Δ32]

General Information

Symbol

Dmel\Cip4^Δ32

Species

D. melanogaster

Name

FlyBase ID

FBal0246556

Feature type

allele

Associated gene

Dmel\Cip4

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Fricke et al., 2009)

Progenitor genotype

P{EPgy2}Cip4^EY10134

(Fricke et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes the first two exons of Cip4, including the start codon.

(Fricke et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Fricke et al., 2009)

visible

(Fricke et al., 2009)

visible (with Df(3L)Exel8098)

(Fricke et al., 2009)

Phenotype Manifest In

adult midgut circular muscle cell

(Schultheis et al., 2019)

adult midgut longitudinal muscle cell

(Schultheis et al., 2019)

egg chamber

(Zobel et al., 2015)

wing hair | increased number

(Zobel et al., 2015, Fricke et al., 2009)

wing hair | increased number (with Df(3L)Exel8098)

(Fricke et al., 2009)

Detailed Description

Statement

Reference

Cip4^Δ32 maternal and zygotic homozygous adults exhibit disrupted longitudinal and circular muscle patterns in midgut (shorter and not arranged strictly in parallel longitudinal muscle fibers with numerous branches -particularly near their ends) compared to controls.

(Schultheis et al., 2019)

Mutants frequently show wing hair duplications.

12.6% of Cip4^Δ32 ovaries have defective egg chambers containing an aberrant number of germline cells.

(Zobel et al., 2015)

Homozygous and Cip4^Δ32/Df(3L)Exel8098 adults show wing hair duplications in about 30% of the wing cells. The orientation of the wing hairs is not affected.

(Fricke et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3L)Exel8098/Cip4^Δ32 has visible phenotype, enhanceable by SCAR^Δ37/SCAR[+]

(Fricke et al., 2009)

NOT Enhanced by

Statement

Reference

Df(3L)Exel8098/Cip4^Δ32 has visible phenotype, non-enhanceable by WASp[+]/WASp³

(Fricke et al., 2009)

NOT suppressed by

Statement

Reference

Df(3L)Exel8098/Cip4^Δ32 has visible phenotype, non-suppressible by WASp[+]/WASp³

(Fricke et al., 2009)

Other

Statement

Reference

Cip4^Δ32, Nost^df004 has partially lethal - majority die phenotype

(Zobel et al., 2015)

Cip4^Δ32, Nost^df004 has female sterile phenotype

(Zobel et al., 2015)

Cip4^Δ32, Nost^df004 has male semi-sterile phenotype

(Zobel et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cip4^Δ32 has adult midgut longitudinal muscle cell phenotype, enhanceable by Nost^df004/Nost^df004

(Schultheis et al., 2019)

Cip4^Δ32 has adult midgut circular muscle cell phenotype, enhanceable by Nost^df004/Nost^df004

(Schultheis et al., 2019)

Cip4^Δ32 has wing hair | increased number phenotype, enhanceable by Nost^df004

(Zobel et al., 2015)

Cip4^Δ32 has egg chamber phenotype, enhanceable by Nost^df004

(Zobel et al., 2015)

Df(3L)Exel8098/Cip4^Δ32 has wing hair | increased number phenotype, enhanceable by SCAR^Δ37/SCAR[+]

(Fricke et al., 2009)

NOT Enhanced by

Statement

Reference

Df(3L)Exel8098/Cip4^Δ32 has wing hair | increased number phenotype, non-enhanceable by WASp[+]/WASp³

(Fricke et al., 2009)

NOT suppressed by

Statement

Reference

Df(3L)Exel8098/Cip4^Δ32 has wing hair | increased number phenotype, non-suppressible by WASp[+]/WASp³

(Fricke et al., 2009)

Other

Statement

Reference

Cip4^Δ32, Nost^df004, Synd^ΔEx22 has embryonic/larval midgut | embryonic stage phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Nost^df004, Synd^ΔEx22 has embryonic somatic muscle cell phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Nost^df004, Synd^ΔEx22 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Nost^df004 has embryonic somatic muscle cell phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Nost^df004 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Synd^ΔEx22 has embryonic/larval midgut | embryonic stage phenotype

(Schultheis et al., 2019)

Cip4^Δ32, Nost^df004 has germarium phenotype

(Zobel et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Cip4^Δ32 Nost^df004 maternal and zygotic homozygous double mutant embryos exhibit somatic muscle defects (missing or small muscle fibers in several segments replaced with mononucleated myoblasts at the corresponding positions) and significantly decreased number of nuclei in muscle DA1 when compared to controls.

Nost^df004 maternal and zygotic Cip4^Δ32 zygotic Synd^ΔEx22 zygotic triple homozygous mutant embryos exhibit somatic muscle defects (missing muscle fibers with unfused myoblasts), significantly decreased number of nuclei in muscle DA1 and slightly expanded midgut anterior chamber when compared to controls.

Cip4^Δ32 Synd^ΔEx22 zygotic homozygous double mutant embryos exhibit slightly expanded midgut anterior chamber compared to controls.

(Schultheis et al., 2019)

44.8% of Cip4^Δ32 Nost^Δ ovaries have defective egg chambers containing an aberrant number of germline cells - they always have two or more oocytes, each oocyte has four ring canals, and oocytes are randomly positioned, indicating a defect in the encapsulation of single germline cysts by follicle cells. Defective egg chambers past stage 9 are not observed. Germarium morphology is clearly affected.

(Zobel et al., 2015)

SCAR^Δ37/+ increases the fraction of wing cells that have multiple wing hairs in Cip4^Δ32/Df(3L)Exel8098 adults.

WASp³/+ has no effect on the fraction of wing cells that have multiple wing hairs in Cip4^Δ32/Df(3L)Exel8098 adults.

(Fricke et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

91557

w^* Nost^df004; Cip4^Δ32 P{EP}Synd^1d/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cip4^Δ32

(Schultheis et al., 2019)

cip4^Δ32

(Fricke et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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