FlyBase Allele Report: Dmel\Rabex-5[VDRC.cUa]

General Information

Symbol

Dmel\Rabex-5^VDRC.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0246621

Feature type

allele

Associated gene

Dmel\Rabex-5

Associated Insertion(s)

Carried in Construct

P{VDRC.Rabex-5.U}

Key Links

Transgenic product class

RNAi_reagent

(Yan et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yan et al., 2010)

Mutagen

in vitro construct

(Yan et al., 2010)

Progenitor genotype

Carried in construct

P{VDRC.Rabex-5.U}

Cytology

Description

UAS regulatory sequences drive expression of an RNAi construct targeting Rabex-5.

(Yan et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rabex-5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased body size, with Scer\GAL4^Act.PU

(Yan et al., 2010)

lethal | larval stage, with Scer\GAL4^unspecified

(Yan et al., 2010)

lethal | P-stage, with Scer\GAL4^unspecified

(Yan et al., 2010)

melanotic mass phenotype | larval stage, with Scer\GAL4^unspecified

(Yan et al., 2010)

melanotic mass phenotype | P-stage, with Scer\GAL4^unspecified

(Yan et al., 2010)

visible, with Rabex-5^ex42, Scer\GAL4^C-765

(Yan et al., 2010)

visible, with Scer\GAL4^Act.PU

(Yan et al., 2010)

visible, with Scer\GAL4^C-765

(Yan et al., 2010)

Phenotype Manifest In

posterior crossvein, with Rabex-5^ex42, Scer\GAL4^C-765

(Yan et al., 2010)

posterior crossvein, with Scer\GAL4^Act.PU

(Yan et al., 2010)

posterior crossvein, with Scer\GAL4^C-765

(Yan et al., 2010)

wing, with Scer\GAL4^Act.PU

(Yan et al., 2010)

wing vein L2, with Rabex-5^ex42, Scer\GAL4^C-765

(Yan et al., 2010)

wing vein L2, with Scer\GAL4^Act.PU

(Yan et al., 2010)

wing vein L2, with Scer\GAL4^C-765

(Yan et al., 2010)

Detailed Description

Statement

Reference

Larvae and pupae expressing Rabex-5^VDRC.cUa driven by strong, constitutive drivers die as giant larvae or giant pupae, often containing melanotic tumors.

Expression of Rabex-5^VDRC.cUa driven by Scer\GAL4^Act.PU results in viable adults with about a 10% increase in length, weight and wing size compared with controls.

Expression of Rabex-5^VDRC.cUa driven by Scer\GAL4^Act.PU or Scer\GAL4^C-765 results in extra posterior crossveins and extra longitudinal veins compared to controls. Rabex-5^ex42/+ enhances the wing phenotype resulting from Rabex-5^VDRC.cUa expression driven by Scer\GAL4^C-765.

(Yan et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+], Scer\GAL4^C-765

(Yan et al., 2010)

Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has visible phenotype, suppressible | partially by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has increased body size phenotype, suppressible | partially by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has visible phenotype, suppressible by Ras85D^e2F/Ras85D[+], Scer\GAL4^Act.PU

(Yan et al., 2010)

Enhancer of

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Egfr^U.A887T.UAS, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT Enhancer of

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Raf^UAS.F179, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Other

Statement

Reference

Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has visible phenotype

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has lethal phenotype

(Yan et al., 2010)

Egfr^U.A887T.UAS, Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU has lethal phenotype

(Yan et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has posterior crossvein phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing vein L2 phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has posterior crossvein phenotype, suppressible by Ras85D^e2F/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing vein L2 phenotype, suppressible by Ras85D^e2F/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has posterior crossvein phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has wing vein L2 phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has eye phenotype, suppressible | partially by Ras85D^e1B/Ras85D[+]

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing phenotype, suppressible | partially by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU

(Yan et al., 2010)

Enhancer of

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Egfr^U.A887T.UAS, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT Enhancer of

Statement

Reference

Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Raf^UAS.F179, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Other

Statement

Reference

Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has eye phenotype

(Yan et al., 2010)

Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has antenna phenotype

(Yan et al., 2010)

Rabex-5^VDRC.cUa, Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has antenna phenotype

(Yan et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rabex-5^VDRC.cUa with Dcr-2^Scer\UAS.cDa in the early eye driven by Scer\GAL4^ey.PU leads to a fraction of the eyes with severe phenotypes, including overgrown eyes, outgrowths, small or lost eyes, and extra antennae.

Ras85D^e1B and Ras85D^e2F dominantly suppress the extra wing veins caused by constitutive expression of Rabex-5^VDRC.cUa via Scer\GAL4^Act.PU.

Ras85D^e1B dominantly suppresses the extra wing veins caused by wing-specific expression of Rabex-5^VDRC.cUa via Scer\GAL4^C-765.

A Ras85D^e1B/+ genetic background results in fewer flies with severe eye phenotypes upon co-expression of Rabex-5^VDRC.cUa and Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^ey.PU.

Ras85D^e1B dominantly suppresses the increased body size, body length and wing area resulting from the expression of Rabex-5^VDRC.cUa driven by Scer\GAL4^Act.PU. Body weight of all flies and wing area in female flies are no longer statistically different from controls.

Co-expression of Rabex-5^VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85D^{V12.S35.Scer\UAS} driven by Scer\GAL4^GMR.PU. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85D^{V12.S35.Scer\UAS} overexpression are also enhanced by Rabex-5^VDRC.cUa-expression.

Co-expression of Rabex-5^VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Egfr^{U.A887T.Scer\UAS} driven by Scer\GAL4^GMR.PU.

Co-expression of Rabex-5^VDRC.cUa fails to enhance the eye phenotypes resulting from the overexpression of phl^{Scer\UAS.F179} driven by Scer\GAL4^GMR.PU.

(Yan et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

This construct is originally from the Vienna Drosophila RNAi Center (VDRC).

(Yan et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rabex-5^VDRC.cUa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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