FlyBase Allele Report: Scer\GAL4[SIFa.PT]

General Information

Symbol

Scer\GAL4^SIFa.PT

Species

S. cerevisiae

Name

IFamide promoter construct of Terhaz

FlyBase ID

FBal0246774

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{SIFa-GAL4.T}

Also Known As

SIFa-GAL4, SIFa2-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Terhzaz et al., 2007)

Progenitor genotype

Carried in construct

P{SIFa-GAL4.T}

Cytology

Description

A 1482bp fragment from immediately 5' of the SIFa coding region regulates expression of a GAL4 driver.

(Terhzaz et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

SIFa

SIFa_1482bp

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval SIFamide cell of pars intercerebralis

(Terhzaz et al., 2007)

adult stage

adult brain | restricted

(Carlsson et al., 2010)

adult pars intercerebralis

(Carlsson et al., 2010)

adult antennal lobe glomerulus | subset

(Carlsson et al., 2010)

adult pars intercerebralis | restricted

(Cavanaugh et al., 2014)

adult SIFamide cell of pars intercerebralis

(Martelli et al., 2017, Terhzaz et al., 2007)

Additional Information

Statement

Reference

Expression of ScerGAL4^SIFa.PT is found in a subset of four ScerGAL4^Kurs58 positive pars intercerebralis (PI) neurons. GFP reconstitution across synaptic partners (GRASP) shows a connection between ScerGAL4^SIFa.PT positive PI neurons and ScerGAL4^Clk.4.1M positive DN1p neurons.

(Cavanaugh et al., 2014)

ScerGAL4^IFa.PT drives expression in 4 cells in the pars intercerebralis that extend processes throughout most of the brain neuropils, including most glmeruli of the antennal lobe. The cells labelled in the pars intercerebralis by ScerGAL4^IFa.PT also express SIFa.

(Carlsson et al., 2010)

Marker for

adult SIFamide cell of pars intercerebralis

(Martelli et al., 2017)

Reflects expression of

SIFa

(Martelli et al., 2017, Cavanaugh et al., 2014, Carlsson et al., 2010, Terhzaz et al., 2007)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 29[o]C) of Scer\GAL4^SIFa.PT cells in sated flies significantly increases preference for appetitive odors and proboscis extension reflex (PER) in response to sucrose, compared to controls; activation has no affect on PER of starved flies or avoidance of aversive odors in sated flies, compared to controls; activation significantly increases short-term and long-term food intake of sated flies, compared to controls, with no effect on locomotor activity; activation of a subset (restricted using Scer\GAL80^tsh-GAL80) of Scer\GAL4^SIFa.PT cells also significantly increases short-term food intake, compared to controls.

Inhibition (using Ork1^{Δ-C.UAS.EGFP}) of Scer\GAL4^SIFa.PT cells, in combination with a Gal80[ts] transgene to restrict expression to during the feeding experiment, does not affect food intake of starved flies, compared to controls.

(Martelli et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^SIFa.PT, TrpA1^{lexAop.mCherry} has abnormal feeding behavior | adult stage | heat sensitive phenotype, suppressible by SIFa^RNAi.UAS.cTa, Scer\GAL4^SIFa.PT

(Martelli et al., 2017)

Scer\GAL4^SIFa.PT, rpr^UAS.cZa has abnormal courtship behavior | male phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^SIFa.PT

(Terhzaz et al., 2007)

Suppressor of

Statement

Reference

SIFa^RNAi.UAS.cTa, Scer\GAL4^SIFa.PT is a suppressor of abnormal feeding behavior | adult stage | heat sensitive phenotype of Scer\GAL4^SIFa.PT, TrpA1^{lexAop.mCherry}

(Martelli et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^SIFa.PT is a suppressor of abnormal courtship behavior | male phenotype of Scer\GAL4^SIFa.PT, rpr^UAS.cZa

(Terhzaz et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^SIFa.PT/Dop2R^UAS.cDa partially rescues Dop2R^attP

(Deng et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

SIFa^Gal4

(Devineni et al., 2013)

Scer\GAL4^IFa.PT

Scer\GAL4^SIFa.PT

Name Synonyms

IFamide promoter construct of Terhaz

Secondary FlyBase IDs

References (20)

Report Sections

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