UAS regulatory sequences drive expression of a mutated form of Hsap\TARDBP which contains the amino acid replacements F147L and F149L in the first RNA recognition motif.
Motor neuron clones expressing Hsap\TARDBPFFLL.Scer\UAS under the control of Scer\GAL4VGlut-OK371 do not exhibit any observable degeneration.
Individuals expressing Hsap\TARDBPFFLL.Scer\UAS under the control of Scer\GAL4elav-C155 exhibit a significant decrease in lifespan as compared to controls.