Amino acid replacement: V478E.
T29120520A
V478E | yem-PA; V478E | yem-PB
V478E
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
female sterile (with yem2)
oocyte (with Df(3R)3450)
Eggs from yemα1/Df(3R)3450 females are normally fertilised and they initiate development, but the embryos die before hatching.
The male pronucleus fails to decondense at the pronuclear apposition stage in eggs derived from yemα1/Df(3R)3450 females. Paternal chromosomes are subsequently excluded from the first zygotic division, resulting in gynogenetic haploid development and embryonic lethality.
70% of eggs laid by homozygous females enter mitotic cycles bu the embryos fail to hatch.
Mature stage S14 yemα1/Df(3R)3450 oocytes show chromosome defects. The symmetry of the congressed chromosome masses relative to the spindle and the symmetry of the congressed chromosome masses relative to the position of the achiasmate 4th chromosomes is affected in the mutant oocytes. The shape of the congressed exchange chromosome mass is also altered in the mutant oocytes, with a higher proportion of monolobed masses.
Df(3R)3450/yem1, mei-9a has abnormal meiotic cell cycle | female phenotype
Df(3R)3450/yem1, mei-W681 has abnormal meiotic cell cycle | female phenotype
mei-W681 ; yemα1/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-W681 single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-W681 single mutants where a greater proportion of the oocytes reach meiosis II).
mei-9a ; yemα1/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-9a single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-9a single mutants where a greater proportion of the oocytes reach meiosis II).
A small but significant proportion of eggs produced by mei-2181/mei-2188 ; yemα1/Df(3R)3450 females are able to develop into adults. These exceptional adults do not display paternal chromosome markers, suggesting that they have developed from diploid gametes that have undergone gynogenesis, a form of parthenogenesis that requires fertilisation.
Df(3R)3450/yem1 is rescued by yemHPF.Tag:FLAG
yemαHPF.T:Zzzz\FLAG rescues the sterility of yemα1/Df(3R)3450 females.