The ple genomic region, including 4.1kb of upstream promoter sequences is cloned (to generate ple+t10.988) and then cassette exons 3 and 4 (also known as c and d) are removed, along with the surround intronic sequences (i[[2]], i[[3]], and i[[4]]).
pleΔ1g fails to rescue ple2
ple2-induced embryonic lethality is not rescued by the presence of pleΔ1g.