UAS regulatory sequences drive expression of an RNAi construct.
Flies expressing G-oα47AVDRC.cUa under the control of Scer\GAL4Gr5a.PUn have a strongly reduced ability to distinguish between 5mM and 2mM sucrose in a 2-choice preference assay compared to wild-type controls.
The L-type sensilla of flies expressing G-oα47AVDRC.cUa under the control of Scer\GAL4Gr5a.PUn show a significantly reduced spike rate in response to sucrose (over a range of concentrations extending from 20 to 500mM) compared to wild-type control sensilla.
The L-type sensilla of 6 day old adults in which G-oα47AVDRC.cUa has been expressed under the control of Scer\GAL4hs.PB using heat shock for 30 minutes (followed by 16 hours recovery at 25[o]C) show a reduced spike rate in response to sucrose (over a range of concentrations extending from 20 to 500mM) compared to control sensilla.
The L-type sensilla of flies expressing G-oα47AVDRC.cUa under the control of Scer\GAL4Gr5a.PUn show a normal electrophysiological response to maltose and trehalose, but show a reduced spike rate in response to glucose or fructose.
Flies expressing G-oα47AVDRC.cUa under the control of Scer\GAL4Gr66a.PD show a normal avoidance response to caffeine and to quinine in a 2-choice preference assay.