Optogenetic activation (using Crei\ChR1ReaChR.UAS.FRT.mCherry.Citrine with 590 nm (yellow) light) of the set of neurons expressing Scer\GAL4NP2631 or Scer\GAL4GMR22H05 that also express Scer\FLP1fru in adult males results in the induction of pulse and sine song in the absence of a female.