FB2024_03 , released June 25, 2024
Allele: Scer\GAL4GMR66C08
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General Information
Symbol
Scer\GAL4GMR66C08
Species
S. cerevisiae
Name
FlyBase ID
FBal0254140
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR66C08-GAL4}
Also Known As
R66C08-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR66C08-GAL4}
Cytology
Description

A 2411 base pair fragment from the flanking non-coding or intronic region of Takr99D is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR66C08
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      mushroom body output neuron 1
      (Otto et al., 2020, Owald et al., 2015)
      PAM01 feedback neuron
      (Ichinose et al., 2021)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR66C08 drives expression in VGlut positive mushroom body medial lobe arborizing neuron 6. EcollexAGMR58E02 positive dopaminergic PAM neurons are in close proximity with ScerGAL4GMR66C08 expressing mushroom body medial lobe arborizing neuron 6 as shown by a positive GFP reconstitution across synaptic partners (GRASP) signal.

      (Owald et al., 2015)
       
      Marker for
      PAM01 feedback neuron
      (Ichinose et al., 2021)
      mushroom body output neuron 1
      (Otto et al., 2020)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Blocking synaptic transmission (using shi1.Scer\UAS at 32[o]C) from Scer\GAL4GMR21D02, Scer\GAL4VT001211, or Scer\GAL4GMR66C08 neurons significantly impairs memory performance compared to controls. With sucrose-reinforced appetitive conditioning or electric shock-reinforced aversive conditioning, immediate memory at 3 minutes post-conditioning is impaired (both training and testing at a restrictive 32[o]C temperature). Appetitive conditioning memory at 24 hours is also impaired (training at a permissive temperature of 23[o]C, testing at the restrictive temperature).

      Blocking synaptic transmission (using shi1.Scer\UAS at 32[o]C) from Scer\GAL4GMR66C08 (but not Scer\GAL4BG01228) neurons significantly abolishes naive odor avoidance behavior (to MCH or OCT at 1:1000 ), compared to controls.

      (Owald et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR66C08
      Name Synonyms
      Secondary FlyBase IDs
        References (14)