FlyBase Allele Report: Dmel\srl[HMS00857]

General Information

Symbol

Dmel\srl^HMS00857

Species

D. melanogaster

Name

FlyBase ID

FBal0257537

Feature type

allele

Associated gene

Dmel\srl

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00857}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00857}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00857

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates high grade glioma

modeled by Hsap\FGFR3::Hsap\TACC3^UAS.cFa

(Frattini et al., 2018)

DOES NOT ameliorate high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Frattini et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Ddc.PU

(Merzetti and Staveley, 2015)

abnormal size | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Basar et al., 2019)

abnormal stress response | oogenesis, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

decreased fecundity | female, with Scer\GAL4^{VP16.mat.αTub67C}

(Basar et al., 2019)

decreased fecundity | female, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Basar et al., 2019)

female semi-sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Basar et al., 2019)

female sterile germline-dependent, with Scer\GAL4^{VP16.mat.αTub67C}

(Basar et al., 2019)

lethal - all die during embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Basar et al., 2019)

long lived, with Scer\GAL4^Ddc.PU

(Merzetti and Staveley, 2015)

visible, with Scer\GAL4^GMR.PU

(Merzetti and Staveley, 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of srl^HMS00857 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to small ovaries; a complete loss of vitellogenic and mature oocytes; a significant reduction in mitochondrial density (ATP-5A) of stage6/7 egg chambers; and in most of ovarioles a single egg chamber at every stage of development through Stage 8 while it does not lead to cell death (Caspase 3 staining) or nuclear membrane degradation (Lamin staining) in Stage 8 egg chambers when compared to controls.

Expression of srl^HMS00857 under the control of Scer\GAL4^{VP16.mat.αTub67C} in stage 6-8 egg chambers leads to failure of oocytes to migrate dorsally, take on yolk and increase in size; and failure of germline cyst ring canals to spread out from each other while the size of egg chamber is increased when compared to controls. It also leads to a double layer of somatic follicle cells overlying the oocytes in stage 8 egg chambers unlike in controls.

(Basar et al., 2019)

At 25[o]C and 29[o]C (for increased expression), expression of srl^HMS00857 driven by Scer\GAL4^GMR.PU decreases the number of ommatidia and bristles in the eye. Expression of srl^HMS00857 driven by Scer\GAL4^Ddc.PU significantly increases lifespan (at either temperature) and leads to mild premature loss in climbing ability (25[o]C) over time compared to controls.

(Merzetti and Staveley, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

srl^HMS00857, Scer\GAL4^how-24B is a suppressor of abnormal locomotor behavior | adult stage phenotype of CG17019^GD1336, Scer\GAL4^how-24B

(Wei et al., 2018)

srl^HMS00857, Scer\GAL4^how-24B is a suppressor of abnormal stress response | adult stage phenotype of CG17019^GD1336, Scer\GAL4^how-24B

(Wei et al., 2018)

srl^HMS00857, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

srl^HMS00857, Scer\GAL4^how-24B is a suppressor of indirect flight muscle cell phenotype of CG17019^GD1336, Scer\GAL4^how-24B

(Wei et al., 2018)

srl^HMS00857, Scer\GAL4^how-24B is a suppressor of mitochondrion | adult stage phenotype of CG17019^GD1336, Scer\GAL4^how-24B

(Wei et al., 2018)

srl^HMS00857, Scer\GAL4^insc-Mz1407 is a suppressor of type II neuroblast | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of srl^HMS00857 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Lee et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33914