FlyBase Allele Report: Dmel\dar1[3232]

General Information

Symbol

Dmel\dar1³²³²

Species

D. melanogaster

Name

FlyBase ID

FBal0258677

Feature type

allele

Associated gene

Dmel\dar1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Ye et al., 2011)

Progenitor genotype

Cytology

Description

dar1³²³² contains a premature stop codon so that only a fragment of the N-terminal half of the protein can be produced, without the nuclear localization signal and zinc finger domains.

(Ye et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Ye et al., 2011)

abnormal neuroanatomy (with dar1³⁰¹⁰)

(Ye et al., 2011)

abnormal neuroanatomy | somatic clone

(Wang et al., 2015)

Phenotype Manifest In

dendritic arborizing neuron

(Ye et al., 2011)

dendritic arborizing neuron (with dar1³⁰¹⁰)

(Ye et al., 2011)

dendritic arborizing neuron | somatic clone

(Wang et al., 2015)

larval multidendritic class III neuron | somatic clone

(Wang et al., 2015)

larval multidendritic class I neuron | somatic clone

(Wang et al., 2015)

larval multidendritic class IV neuron | somatic clone

(Wang et al., 2015)

Detailed Description

Statement

Reference

dar1³²³²/dar1³²³² larval dendritic arborizing neurons display loss of the multipolar layout of dendrites, with many becoming bipolar, and some Class I dendritic arborizing neurons becoming unipolar. Axon targeting and terminal branching is unaffected.

dar1³²³²/dar1³²³² larval es neurons not do not show a significant change in morphology as compared to wild type.

(Wang et al., 2015)

dar1³²³² engenders a decrease in the percentage of multi-compartmental Golgi in dendrites of larval dendritic arborization neurons without affecting the Golgi in the soma and branch points.

(Zhou et al., 2014)

In dar1³²³² mutants, major dendrites of class IV da neurons are either missing or shortened, consequently resulting in a reduced dendritic arbor. In contrast, the axonal growth of these neurons is normal. Dendrites of class I da neurons are also severely shortened.

dar1³²³²/dar1³⁰¹⁰ mutants, generated by crossing dar1³²³² germline clones with dar1³⁰¹⁰ mutants show the same phenotype as dar1³²³² zygotic mutants, that is, the dendrites are reduced but the axons are normal. Dendritic defects are visible as early as 16-17 hours after egg laying and become more pronounced in 18-19 hours after egg laying, suggesting these mutants exhibit reduced outgrowth of dendrites.

Single neurons homozygous for dar1³²³², generated through MARCM, exhibit severely reduced dendritic arbors, supporting the notion that dar1 functions cell-autonomously. Dendrites are dramatically reduced in all four classes of da neurons (class I-ddaD and ddaE, class II-ddaB, class III-ddaA, and class IV-ddaC). In contrast, there is no significant defect in axonal growth in these mutants.

(Ye et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dar1³²³² has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Scer\GAL4^unspecified/nudE^UAS.A

(Wang et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

dar1³²³² has dendritic arborizing neuron | somatic clone phenotype, suppressible | partially by Scer\GAL4^unspecified/nudE^UAS.A

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of nudE^Scer\UAS.A under the control of Scer\GAL4^unspecified partially rescues the increased number of bipolar dendritic arborizing neurons seen in dar1³²³²/dar1³²³² mutant clones.

(Wang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dar1³²³² is rescued by dar1^+tYa

(Ye et al., 2011)

Comments

The presence of dar1^+tYa rescues the dendrite defects in both class IV and class I neurons mutant for dar1³²³².

(Ye et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65260

w^*; dar1³²³² P{FRT(w^hs)}2A/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dar1³²³²

(Wu et al., 2018, Wang et al., 2015, Zhou et al., 2014, Ye et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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