FB2024_03 , released June 25, 2024
Allele: Dmel\NetBtm
Open Close
General Information
Symbol
Dmel\NetBtm
Species
D. melanogaster
Name
FlyBase ID
FBal0260876
Feature type
allele
Associated gene
Dmel\NetB
Associated Insertion(s)
TI{TI}NetBtm
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Brankatschk and Dickson, 2006)
Progenitor genotype
Associated Insertion(s)
TI{TI}NetBtm
Cytology
Description

A transmembrane domain (Tag:TM(Synth)) flanked by Tag:V5 (intracellular) and Tag:MYC (extracellular) epitope tags has been introduced to the C-terminus of NetB. The transmembrane domain is expected to tether the NetB protein to the cell membrane.

(Brankatschk and Dickson, 2006)
Allele components
Component
Use(s)
Tagged with
Tag:MYC
Tag:TM(Synth)
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 16
      embryonic/larval dorsal vessel | restricted
      (Asadzadeh et al., 2016)
      embryonic cardioblast

      Comment: expressed in tin-positive cardioblasts

      (Asadzadeh et al., 2016)
      Additional Information
      Statement
      Reference

      The tagged membrane-bound NetB is homogenously expressed in all tin-positive cardoblast membranes but not in tinFBtr0091350:pb-XRsvp-expressing cells along the dorsal vessel.

      (Asadzadeh et al., 2016)
       
      Marker for
      Reflects expression of
      NetB
      (Asadzadeh et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A small percentage of R8 axons in NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC mutants project abnormally, past the distal medulla neuropil border at 24 and 42 hour after pupal formation. Projections are unaffected at 55 hours and in adults.

      (Timofeev et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The anterior commissure appears normal in all segments and the posterior commissure appears normal in most of the segments in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC double mutant embryos. Similarly to wild-type, both the EW and EG axons cross the midline in every segment of NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos.

      NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC animals are viable and fertile with no obvious morphological phenotype.

      Short-range repulsion is preserved in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL41407, as the ventral nerve cord commissures are completely absent in every segment examined.

      Long-range axon-repulsion is completely abolished in NetAΔ, NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC embryos that express unc-5Scer\UAS.cKa under the control of Scer\GAL4ato-NP6558.

      (Brankatschk and Dickson, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      NetBKI.T:SV5\V5,T:Zzzz\TM,T:Hsap\MYC
      NetBTM
      (Timofeev et al., 2012)
      NetBmyc-TM
      (von Hilchen et al., 2010)
      NetBtm
      (Kang et al., 2019, Brankatschk and Dickson, 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)