FlyBase Allele Report: Dmel\sqh[DD.UAS]

General Information

Symbol

Dmel\sqh^DD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0263515

Feature type

allele

Associated gene

Dmel\sqh

Associated Insertion(s)

Carried in Construct

P{UAS-sqh.DD}

Also Known As

UAS-sqh^DD, UAS-Sqh^D20D21

Key Links

Transgenic product class

missense_variant

(Mitonaka et al., 2007)

polypeptide_post_translational_processing_variant

(Mitonaka et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mitonaka et al., 2007)

polypeptide_post_translational_processing_variant

(Mitonaka et al., 2007)

Mutagen

in vitro construct

(Jiang et al., 2011, Mitonaka et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-sqh.DD}

Cytology

Description

UASt regulatory sequences drive expression of an activated form of sqh carrying amino acid substitutions T20E and S21E to mimic the constitutive phosphorylated form.

(Mitonaka et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sqh

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates high grade glioma

modeled by btl::Egfr^λ.UAS

(Chen et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | adult stage, with Scer\GAL4^NP7397

(Bohere et al., 2022)

Phenotype Manifest In

adult gut, with Scer\GAL4^NP7397

(Bohere et al., 2022)

eye disc, with Scer\GAL4^ey.PU

(Jiang et al., 2011)

eye disc | somatic clone, with Scer\GAL4^Act.PU

(Jiang et al., 2011)

wing disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Mitonaka et al., 2007)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Mitonaka et al., 2007)

wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mitonaka et al., 2007)

Detailed Description

Statement

Reference

Cells from clones in the eye disc expressing sqh^DD.Scer\UAS under the control of Scer\GAL4^Act.PU become mislocalised in the optic stalk.

Cells from the eye disc expressing sqh^DD.Scer\UAS under the control of Scer\GAL4^ey.PU become mislocalised in the optic stalk.

(Jiang et al., 2011)

Wing discs overexpressing sqh^DD.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 are multi-layered and show malformed disc shapes.

In contrast to the columnar wild-type cells, wing disc cells in clones overexpressing sqh^DD.Scer\UAS driven by Scer\GAL4^Act5C.PI shrink basally and show abnormal apical-basal polarity.

In contrast to wild-type controls, cells overexpressing sqh^DD.Scer\UAS under the control of Scer\GAL4^ptc-559.1 appear to migrate out from the Scer\GAL4^ptc-559.1-expression domain.

(Mitonaka et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^repo.PU/sqh^DD.UAS is an enhancer of hyperplasia | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Scer\GAL4^repo.PU/sqh^DD.UAS is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Other

Statement

Reference

Scer\GAL4^repo.PU, btl::Egfr^λ.UAS, sqh^DD.UAS has neoplasia | third instar larval stage phenotype

(Chen et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^repo.PU/sqh^DD.UAS is an enhancer of embryonic/larval brain | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Scer\GAL4^repo.PU/sqh^DD.UAS is an enhancer of larval brain neuropil glial cell | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

sqh^DD.UAS/Scer\GAL4^c381 is an enhancer of embryonic/larval cuticle | embryonic stage phenotype of α-Cat¹

(Jurado et al., 2016)

sqh^DD.UAS/Scer\GAL4^c381 is an enhancer of embryonic/larval cuticle | embryonic stage phenotype of α-Cat¹³

(Jurado et al., 2016)

NOT Enhancer of

Statement

Reference

sqh^DD.UAS/Scer\GAL4^c381 is a non-enhancer of embryonic/larval cuticle | embryonic stage phenotype of α-Cat²⁰⁴⁹

(Jurado et al., 2016)

sqh^DD.UAS/Scer\GAL4^c381 is a non-enhancer of embryonic/larval cuticle | embryonic stage phenotype of α-Cat⁴²¹

(Jurado et al., 2016)

Suppressor of

Statement

Reference

sqh^DD.UAS/Scer\GAL4^Act5C.PP is a suppressor of fat body | somatic clone | calorie restriction conditional phenotype of Scer\GAL4^Act5C.PP, sqa^RNAi.UAS

(Tang et al., 2011)

sqh^DD.UAS/Scer\GAL4^Act5C.PP is a suppressor of autophagosome | somatic clone | calorie restriction conditional phenotype of Scer\GAL4^Act5C.PP, sqa^RNAi.UAS

(Tang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing sqh^DD.UAS enhances the larval brain glia hyperplasia induced by the expression of btl::Egfr^λ.UAS under the control of Scer\GAL4^repo.PU; glia cells lose the normal stellate shape and form abnormal cellular aggregates that disrupted normal larval brain architecture, indicative of neoplastic transformation.

(Chen et al., 2019)

The severity of the embryonic cuticle defects in α-Cat¹ or α-Cat¹³ mutants but not in α-Cat⁴²¹ or α-Cat²⁰⁴⁹ embryos can be significantly aggravated by expression of sqh^DD.Scer\UAS under the control of Scer\GAL4^c381 in the mutant background.

(Jurado et al., 2016)

Co-expression of sqh^DD.Scer\UAS with sqa^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP suppresses the sqa^{dsRNA.Scer\UAS}-induced autophagic defects in larval fat body cells.

(Tang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600573

w^*; P{UAS-sqh.DD}C1/CyO, P{Dfd-EYFP.w^BmC}2; TM2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sqh^D20D21

(Mitonaka et al., 2007)

sqh^DD.Scer\UAS

sqh^DD.UAS

sqhDD

(Jurado et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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