FlyBase Allele Report: Dmel\Utx[1]

General Information

Symbol

Dmel\Utx¹

Species

D. melanogaster

Name

FlyBase ID

FBal0264573

Feature type

allele

Associated gene

Dmel\Utx

Associated Insertion(s)

Carried in Construct

Also Known As

dUTX¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhang et al., 2013)

hypomorphic allele - molecular evidence

(Herz et al., 2010)

Mutagen

ethyl methanesulfonate

(Herz et al., 2010)

Progenitor genotype

Cytology

Description

A premature termination codon in the JmjC domain.

(Herz et al., 2010)

Amino acid replacement: ?966term.

(Herz et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,273,390..10,273,390 [-]

Nucleotide change:

C10273390T

Amino acid change:

Reported amino acid change:

Q966term

Comment:

The position of the mutation was reported relative to isoform Utx-PA. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Herz et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Pinzón et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by lola^GS88A8, psq^GS88A8

(Herz et al., 2010)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Song et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Shalaby et al., 2017)

lethal - all die during pupal stage

(Denton et al., 2013)

partially lethal (with Utx^Δ95)

(Zhang et al., 2013)

partially lethal - majority die | recessive

(Herz et al., 2010)

radiation sensitive (with Utx^Δ95)

(Zhang et al., 2013)

short lived (with Df(2L)BSC143)

(Copur and Müller, 2013)

Phenotype Manifest In

embryonic/larval salivary gland

(Denton et al., 2013)

eye

(Herz et al., 2010)

eye | somatic clone

(Herz et al., 2010)

eye-antennal disc | somatic clone

(Herz et al., 2010)

sex comb (with Utx^f01321)

(Herz et al., 2010)

wing (with Utx^f01321)

(Herz et al., 2010)

Detailed Description

Statement

Reference

Utx¹ mutants are homozygous lethal.

(Shalaby et al., 2017)

The majority of Utx¹/Df(2L)BSC143 animals that are derived from Utx¹/+ females develop into adults that are morphologically indistinguishable from wild type, but die shortly after eclosion.

Homozygotes only rarely survive to adulthood.

Homozygous clones in the eye-antennal imaginal disc do not show an overproliferation phenotype.

(Copur and Müller, 2013)

The normal degradation of salivary glands is significantly delayed in Utx¹ mutants - intact glands are still present in many animals at 24 h relative to puparium formation.

Utx¹ homozygotes survive past the head eversion stage but die before eclosion.

Utx¹ salivary glands show reduced caspase activity and reduced autophagy, compared to controls.

(Denton et al., 2013)

Utx^Δ95/Utx¹ animals can develop into adults, which show no detectable morphological defects.

Utx^Δ95/Utx¹ embryos show a markedly lower hatching rate compared to wild type. Treatment with 10 Gy of ionizing radiation reduces the hatching rate of Utx^Δ95/Utx¹ embryos to a greater extent than that of wild type. In contrast, the hatching rate of mutant embryos is not significantly different to wild type after UV radiation exposure.

(Zhang et al., 2013)

The overall size of Utx¹ mosaic eyes is normal; however, Utx¹ mutant tissue represents a larger fraction of the eye. This overrepresentation phenotype is already apparent in mosaic eye-antennal imaginal discs.

While most homozygotes die during pupal stages, a small percentage (< 5%) develop as viable adults. Nevertheless, these homozygous escapers die immediately after eclosion.

The sex combs of Utx¹/Utx^f01321 males contain fewer teeth (6-8) than wild type (~10).

Homozygotes have rough eyes.

Utx¹/Utx^f01321 wings sometimes contain fluid-trapped blisters.

No significant change in apoptosis is observed for Utx¹ mosaics during eye imaginal disc development.

(Herz et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Utx¹ has lethal - all die during pupal stage phenotype, enhanceable by EcR^RNAi.UAS.cCa/Scer\GAL4^Sgs3.PD

(Denton et al., 2013)

Suppressed by

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has abnormal size | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

Enhancer of

Statement

Reference

Utx[+]/Utx¹ is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Utx[+]/Utx¹ is an enhancer of abnormal size phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Herz et al., 2010)

Other

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has abnormal size | somatic clone phenotype

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has partially lethal - majority die | somatic clone phenotype

(Herz et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^Tub.PU, Utx¹ has abnormal size | somatic clone phenotype

(Herz et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Utx¹ has eye | somatic clone phenotype, enhanceable by Rbf^Δ14/Rbf[+]

(Herz et al., 2010)

Suppressed by

Statement

Reference

Utx¹ has eye | somatic clone phenotype, suppressible by E(z)[+]/E(z)⁷³¹

(Herz et al., 2010)

Utx¹ has eye | somatic clone phenotype, suppressible by Pc¹/Pc[+]

(Herz et al., 2010)

Utx¹ has eye | somatic clone phenotype, suppressible by N[+]/N^264-39

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has adult head | somatic clone phenotype, suppressible by Rbf^UAS.cDa, Scer\GAL4^Tub.PU

(Herz et al., 2010)

Enhancer of

Statement

Reference

Utx[+]/Utx¹ is an enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Utx[+]/Utx¹ is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Herz et al., 2010)

Suppressor of

Statement

Reference

Utx[+]/Utx¹ is a suppressor of wing margin phenotype of Ser¹

(Herz et al., 2010)

Other

Statement

Reference

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype

(Herz et al., 2010)

Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹ has adult head | somatic clone phenotype

(Herz et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype

(Herz et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel is increased by combination with a single copy of Utx¹.

(Shalaby et al., 2017)

Scer\GAL4^Sgs3.PD-mediated expression of EcR^{dsRNA.Scer\UAS.cCa} in a Utx¹ background significantly reduces the proportion of animals surviving to head eversion, compared to Utx¹ animals alone.

(Denton et al., 2013)

Heterozygosity for E(z)⁷³¹ or Pc¹ or N^264-39 suppresses the over-representation phenotype of Utx¹ mosaics.

The wing notching phenotype of Ser¹ or Df(1)N-8heterozygotes is dominantly suppressed by Utx¹.

Scer\GAL4^ey.PH-mediated expression of Dl^Scer\UAS.cDa, psq^GS88A8 and lola^GS88A8 causes mild overgrowth of the eye (the 'eyeful' phenotype) at 18[o]C. This phenotype is enhanced by heterozygosity for Utx¹ : approximately 80% of flies show enlarged ventral halves of the eyes, while the remaining 20% have ventrally located outgrowths and ectopic eye tissue.

MARCM clones of Dl^Scer\UAS.cUa in Utx¹ mosaic eyes result in massive overgrowth of the adult eye and head. The overgrowth is so strong that only a few mosaic flies are recovered as adults; most die at pupal stages.

MARCM clones of Ras85D^{V12.S35.Scer\UAS} in Utx¹ mosaic eyes result in mild overgrowth of the adult eye.

Heterozygosity for Rbf^Δ14 strongly enhances the over-representation phenotype of Utx¹ mosaic eyes: all mosaic eyes are now composed of almost 100% Utx¹ tissue.

(Herz et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Utx¹ is rescued by Scer\GAL4^Tub.PU/Utx^UAS.cHa

(Herz et al., 2010)

Partially rescued by

Utx¹ is partially rescued by Scer\GAL4^Sgs3.PD/Utx^UAS.cHa

(Denton et al., 2013)

Not rescued by