Viable homozygous Ime4c1 adults are recovered at much lower than expected Mendelian frequencies and are significantly subfertile. Non-viable homozygotes die at larval and pupal stages.
Compound egg chambers with supernumerary nurse cells are observed in the ovaries of the rare Ime4c1 homozygous mutant adults. Ovarioles containing such egg chambers rarely mature past mid-oogenesis; egg chambers at later stages frequently degenerate with pyknotic nuclei. Even seemingly normal ovarioles in the mutants display a high frequency of degenerating egg chambers, and only 10% have mature egg chambers in vitellogenic stages.
Mutant follicles contain two or more oocytes that fail to localize to the posterior end. Nearly all oocytes in compound follicles are surrounded by the wild type number of four ring canals. The number of nurse cells in compound follicles usually corresponds to a multiple of the number of oocytes. Synaptonemal complex formation is observed in Ime4c1 oocytes, indicating they enter meiosis.
A common feature of mutant germaria is the presence of two or more partially encapsulated germline cysts on the same plane, instead of a single cyst formed upon encapsulation as seen in controls. In egg chambers that emerge from these germaria, follicle cells are disorganized, while specialized follicle cell types, such as polar cells, are frequently absent or mislocalized. Stalks are wider than normal or missing completely. In the most severe cases, there is a continuum of germline cysts from germaria to subsequent maturing stages with no apparent cyst individualization.
Mettl3c1 has egg chamber phenotype, suppressible by Nicd.hs
Expression of Nicd.hs by performing daily heat-shocks for 5 days of adulthood significantly reduces the frequency of compound egg chamber formation in Ime4c1 mutant ovaries and allows progression through oogenesis past the previtellogenic stages. Significant suppression is even observed in the absence of heat shock, probably because of leaky expression of Nicd.hs.
Mettl3c1 is rescued by Scer\GAL4Act5C.PU/Mettl3UASp.cHa
Mettl3c1 is not rescued by Scer\GAL4Act5C.PU/Mettl3UASp.MUT
The semi-lethal, semi-fertile and egg chamber phenotypes of Ime4c1 are rescued by expressing Ime4Scer\UAS.P\T.cHa under the control of Scer\GAL4Act5C.PU.
The semi-lethal phenotype of Ime4c1 is not rescued by expressing Ime4Scer\UAS.P\T.MUT under the control of Scer\GAL4Act5C.PU.