FB2024_03 , released June 25, 2024
Allele: Hsap\HTTQ15.UAS.mRFP1
Open Close
General Information
Symbol
Hsap\HTTQ15.UAS.mRFP1
Species
H. sapiens
Name
FlyBase ID
FBal0267404
Feature type
allele
Associated gene
Hsap\HTT
Associated Insertion(s)
Carried in Construct
P{UAS-HTT.Q15.mRFP}
Also Known As
UAS-HttQ15-mRFP, UAS-RFP-HttQ15, UAS-HTT15Q-mRFP
Key Links
Transgenic product class
inframe_deletion
(Weiss et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Weiss et al., 2012)
Mutagen
in vitro construct
(Weiss et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-HTT.Q15.mRFP}
Cytology
Description

UASt sequences drive expression of the N terminal 588 amino acids of Hsap\HTT cDNA containing a non-pathogenic tract of 15 polyQ repeats. The construct is tagged at the N-terminal end with mRFP1.

(Weiss et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\HTT
Tagged with
mRFP1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      DOES NOT model  Huntington's disease
      CEA
      (Weiss and Littleton, 2016)
      model of  Huntington's disease
      CEA
      (White et al., 2020)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Huntington's disease
      is exacerbated by Hip1MI05905
      (White et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\HTTQ15.Scer\UAS.T:Disc\RFP-mRFP with a weak elav-GAL4 driver (Scer\GAL4elav.PLu) results in viable adults that appear behaviourally normal at the time of eclosion and do not display any motor coordination or grooming behaviour defects.

      No aggregates are formed when Hsap\HTTQ15.Scer\UAS.T:Disc\RFP-mRFP is expressed under the control of Scer\GAL4elav-C155 in third instar larvae. The Hsap\HTT protein remains diffuse throughout the cytoplasm and neurites.

      (Weiss et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\HTTQ15.Scer\UAS.T:Disc\RFP-mRFP
      Hsap\HTTQ15.UAS.mRFP1
      Name Synonyms
      Secondary FlyBase IDs
        References (12)