UAS regulatory sequences drive expression of Hsap\MECP2.
eye, with Scer\GAL4GMR.PS
Expression of Hsap\MECP2Scer\UAS.cLa in the eye under the control of Scer\GAL4GMR.PS engenders a rough-eye phenotype.
Expression of Hsap\MECP2Scer\UAS.cLa in the wing pouch under the control of Scer\GAL4Bx-MS1096 leads to the formation of ectopic vein tissue near L3, L4 and L5.
Expression of Hsap\MECP2Scer\UAS.cLa under the control of Scer\GAL4da.G32 results in the inhibition of larval growth and the animals die showing signs of melanization in the caudal region at the time of pupariation (180 hours after egg laying at 25[o]C). Animals expressing Hsap\MECP2Scer\UAS.cLa via Scer\GAL4da.G32 develop less severe phenotypes at 18[o]C. Although there are some escapers, these flies display defects, such as smaller size compared with wild-type, extra bristles in the scutellum region compared with four large macrochaetae present the wild-type.
Expression of Hsap\MECP2Scer\UAS.cLa under the control of Scer\GAL4da.G32 and Scer\GAL80ts.αTub84B engenders increased proliferation of intestinal stem cells compared with controls. Consistent with the increase in cell proliferation, the total number of cells in the midgut is increased by 1.6-fold compared with controls.
While wild-type guts exhibit a single layer of epithelium, the guts expressing Hsap\MECP2Scer\UAS.cLa in enterocytes under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B show intestinal hyperplasia with multi-layered epithelium. Compared with wild-type, no significant change in apoptosis or nuclear morphology is observed as a result of Hsap\MECP2Scer\UAS.cLa expression.