UASt regulatory sequences drive expression of full-length Scr containing the mutation E19G. The protein is tagged at the N-terminal end with C-Venus, a non-fluorescent split fluorescent protein fragment. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.
Co-expression of ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4hs.PB does not result in the formation of ectopic salivary glands in the embryo.
Co-expression of ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VN and ScrFL.E19G.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4nullo.PG does not result in the transformation of mesothoracic and metathoracic segments towards prothoracic segments in the embryo.
Construct for BiFC analysis (reconstitution of YFP fluorescence occurs when the T:Avic\GFPVC tag in this construct is brought together in the cell with a protein containing the T:Avic\GFPVN tag).