UASt regulatory sequences drive expression of a synthetic version of Scr consisting of the YPWM motif, the homeodomain and the small C-terminus. The protein is tagged at the N-terminal end with N-Venus, a non-fluorescent split fluorescent protein fragment. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.
Co-expression of ScrSynth.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4hs.PB results in the formation of ectopic salivary glands in the embryo.
Co-expression of ScrSynth.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4nullo.PG results in the transformation of mesothoracic and metathoracic segments towards prothoracic segments in the embryo, as shown by the presence of ectopic beards.
Co-expression of ScrSynth.Scer\UAS.N.T:Avic\GFP-VN and ScrSynth.Scer\UAS.N.T:Avic\GFP-VC under the control of Scer\GAL4Dll-md23 results in complete transformation of antenna to tarsal segments.
Construct for BiFC analysis (reconstitution of YFP fluorescence occurs when the T:Avic\GFPVN tag in this construct is brought together in the cell with a protein containing the T:Avic\GFPVC tag).