FlyBase Allele Report: Dmel\Ack[KD.UAS]

General Information

Symbol

Dmel\Ack^KD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0269478

Feature type

allele

Associated gene

Dmel\Ack

Associated Insertion(s)

Carried in Construct

P{UAS-Ack.KD}

Also Known As

UAS-KD-DACK

Key Links

Transgenic product class

missense_variant

(Sem et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Sem et al., 2002)

Mutagen

in vitro construct

(Sem et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Ack.KD}

Cytology

Description

UASt regulatory sequences drive expression of a kinase dead version of Ack (contains the amino acid replacement K156R).

(Sem et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ack

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

some die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

visible, with Scer\GAL4^71B

(Sem et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

Phenotype Manifest In

embryo | dorsal closure stage | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

embryonic/first instar larval cuticle | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

embryonic head | heat sensitive, with Scer\GAL4^hs.PB

(Sem et al., 2002)

eye, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Sem et al., 2002)

wing, with Scer\GAL4^71B

(Sem et al., 2002)

wing vein | ectopic, with Scer\GAL4^71B

(Sem et al., 2002)

Detailed Description

Statement

Reference

Embryos expressing Ack^KD.Scer\UAS under the control of Scer\GAL4^ptc-559.1 are highly disorganised.

Expression of Ack^KD.Scer\UAS under the control of Scer\GAL4^hs.PB in 6 to 12 hour embryos using a 1 hour heat shock results in approximately half the embryos failing to survive to the first larval instar stage. Of the dead embryos, 34% have germband retraction failures, 15% have holes in the dorsal cuticle (indicating failure of dorsal closure) and 35% do not secrete any cuticle. In addition, most of the embryos with dorsal holes also have head defects.

Flies expressing Ack^KD.Scer\UAS under the control of Scer\GAL4^71B have rounded, spread-out wings with frequent blisters and ectopic veins.

Flies expressing Ack^KD.Scer\UAS under the control of Scer\GAL4^GMR.PF have rough eyes which are smaller than normal. The eyes almost completely lack ommatidia. Bristles are missing in the posterior two-thirds of the eye, while clusters of bristles are present at the anterior end.

(Sem et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ack^KD.Scer\UAS

Ack^KD.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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