Insertion that is predicted to result in truncation within the PDZ domain, evenly disrupting most isoforms. The splice trap within the insertion is not fully efficient, as some residual protein can be detected.
myofibril (with Df(2R)BSC427)
myofibril | adult stage (with Df(2R)BSC427)
Z disc (with Df(2R)BSC427)
Z disc | adult stage (with Df(2R)BSC427)
The indirect flight muscles of Zasp52MI02988/Df(2R)BSC427 transheterozygotes do not show changes in Z-disc size, as compared to controls.
Zasp52MI02988/Zasp52MI02988 mutants exhibit lethality at all developmental stages and only 20% adult escapers. Zasp52MI02988/Zasp52MI02988 and Zasp52MI02988/Df(2R)BSC427 flies show indirect flight muscle defects, as compared to controls. Zasp52MI02988/Df(2R)BSC427 myofibril assembly in indirect flight muscle is severely disrupted, with Z-discs and M-lines no longer distinguishable in many myofibrils, as compared to controls.
Zasp52MI02988/+ mutants do not show obvious morphological defects of the indirect flight muscle, and do not show a significant difference in wing beat frequency, as compared to wild type.
Zasp52MI02988/Zasp52[+] is a suppressor of abnormal flight phenotype of Actn8
Zasp52MI02988 is an enhancer of myofibril phenotype of Act88F6
Zasp52MI02988 is an enhancer of sarcomere phenotype of Act88F6
Zasp52MI02988 is an enhancer of indirect flight muscle cell phenotype of Act88F6
Zasp52MI02988/+ suppresses the decreased wing beat frequency observed in Actn8/+ mutants, but Zasp52MI02988/+, Actn8/+ mutants do not show obvious morphological defects of the indirect flight muscle, as compared to wild type.
Zasp52MI02988/Df(2R)BSC427 is rescued by Zasp52ΔZM.UAS.PR.Tag:FLAG/Scer\GAL4Hk-UH3
Zasp52MI02988/Df(2R)BSC427 is rescued by Zasp52UAS.PK.FSH/Scer\GAL4Hk-UH3
Zasp52MI02988/Df(2R)BSC427 is partially rescued by Zasp52Stop143.UAS.Tag:FLAG/Scer\GAL4Hk-UH3
Zasp52MI02988/Df(2R)BSC427 is not rescued by Zasp52ΔPDZ.UAS.PR.Tag:FLAG/Scer\GAL4Hk-UH3
Zasp52MI02988/Df(2R)BSC427 is not rescued by Zasp52LIM234.UAS.FSH/Scer\GAL4Hk-UH3
Expression of Zasp52Scer\UAS.PK.FSH, Zasp52ΔZM.Scer\UAS.PR.T:Zzzz\FLAG, or Zasp52Scer\UAS.PR.T:Zzzz\FLAG, but not Zasp52LIM234.Scer\UAS.FSH or Zasp52ΔPDZ.Scer\UAS.PR.T:Zzzz\FLAG under the control of Scer\GAL4Hk-UH3 fully rescues the indirect flight muscles seen in Zasp52MI02988/Df(2R)BSC427 mutants.