FlyBase Allele Report: Scer\GAL4[ppk23.2.695]

General Information

Symbol

Scer\GAL4^ppk23.2.695

Species

S. cerevisiae

Name

FlyBase ID

FBal0277044

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ppk23-Gal4.2.695}

Also Known As

ppk23-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Thistle et al., 2012)

Progenitor genotype

Carried in construct

P{ppk23-Gal4.2.695}

Cytology

Description

A 2.695kb upstream fragment of ppk23 regulates expression of a GAL4 driver.

(Thistle et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

ppk23

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

gustatory receptor neuron of terminal organ dorsolateral group

(Mast et al., 2014)

gustatory receptor neuron of terminal organ distal group | subset

(Mast et al., 2014)

Additional Information

Statement

Reference

Drives expression in calcium-sensing neuron of labellar S-type taste bristles, and in a subset of leg taste bristle chemosensory neurons that project into the adult ventral nerve cord; and in gustatory receptor neurons of the terminal organ in larvae.

(FlyBase Curators, 2017)

Scer\GAL4^ppk23.2.695 drives expression in 10 neurons that innervate the terminal organ, and in one internal sensory neuron located along the larval pharynx.

(Mast et al., 2014)

ScerGAL4^ppk23.2.695 drives expression in gustatory receptor neurons in the proboscis and in the legs. These neurons labeled with ScerGAL4^ppk23.2.695 do not overlap with expression of ScerGAL4^ppk28.PC, which labels the water-sensing neurons, ScerGAL4^Gr64f.9.7 which labels the sugar-responsive neurons,or with candidate pheromone receptor ScerGAL4^Gr32a.3.776. A few positive ScerGAL4^ppk23.2.695 neurons in the proboscis co-express AvicGFP^{Gr66a.3x.IRES.2x} which labels bitter-responsive neurons, but there is no co-expression in the leg sensory neurons. Expression of ScerGAL4^ppk23.2.695 in the legs marks more than one cell per bristle and it overlaps with that of fru^P1.LexA.

(Thistle et al., 2012)

There is sexual dimorphism in the leg gustatory receptor neurons labeled by ScerGAL4^ppk23.2.695 : twice as many cells are labeled in males compared to females and the neuronal projections of males in the prothoracic ganglion cross the midline whereas female ones do not.

(Thistle et al., 2012)

Marker for

Reflects expression of

ppk23

(Clowney et al., 2015, Shankar et al., 2015, Thistle et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

abnormal chemosensory behavior | larval stage, with Ctet\tetX^TNT-LC.UAS

(Mast et al., 2014)

abnormal courtship behavior, with Ctet\tetX^TNT-LC.UAS

(Thistle et al., 2012)

abnormal courtship behavior, with fru^RNAi.UAS.1

(Chen et al., 2021)

abnormal courtship behavior | male limited | heat sensitive, with Ecol\lexA^{VGlut-MI04979-TlexA:QFAD.2}, Scer\GAL80^lexAop.cTa, TrpA1^UAS.(B).cKa

(Kallman et al., 2015)

abnormal locomotor behavior | larval stage, with Ctet\tetX^TNT-LC.UAS

(Mast et al., 2014)

abnormal neuroanatomy | adult stage | female, with robo1^GD2608

(Sato et al., 2020)

abnormal neuroanatomy | adult stage | female, with tei^RNAi.ex4.UAS

(Sato et al., 2020)

abnormal neuroanatomy | adult stage | female, with tei^{RNAi.ex6-7.UAS}

(Sato et al., 2020)

abnormal neuroanatomy | adult stage | male, with tei^UAS.Tag:HA

(Sato et al., 2020)

abnormal neurophysiology | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

Phenotype Manifest In

short labellar taste bristle | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

Detailed Description

Statement

Reference

Inactivation of a subset of Scer\GAL4^ppk23.2.695 cells (using Ctet\tetX^{TNT-LC.FRT.stop.UAS} and Scer\FLP1³³⁵) in males has little effect on the frequency of successful matings, copulation duration or copulation latency, compared to controls.

(Jois et al., 2018)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of a subset of Scer\GAL4^ppk23.2.695 cells (activation restricted using Ecol\lexA^{VGlut-MI04979-TlexA:QFAD.2} and Scer\GAL80^{ts.lexAop.cTa}) in adult males significantly decreases wing extensions towards females, compared to controls.

(Kallman et al., 2015)

When males flies in which the Scer\GAL4^ppk23.2.695-expressing neurons have been silenced are exposed to other males that have been engineered to produce female pheromones (by expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^OK72) they do not show the increase in starvation resistance seen when they are exposed to wild type males.

Activation of Scer\GAL4^ppk23.2.695-expressing neurons in wild type males results in reductions in starvation resistance, triacylglyceride (TAG) amount and lifespan compared with wild type males. Activity levels, respiration rate and feeding are unaffected.

(Gendron et al., 2014)

Inhibition (using Ctet\TeTxLC^TNT.UAS) of Scer\GAL4^ppk23.2.695 cells significantly reduces the preference of larvae for larval-treated substrate, compared to controls; attraction to the secreted larval fatty acids (Z)-5-tetradecanoic acid and (Z)-7-tetradecanoic acid is significantly reduced, compared to controls.

(Mast et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ppk23.2.695, tei^RNAi.ex4.UAS has abnormal neuroanatomy | adult stage | female phenotype, enhanceable by robo1^GD2608/Scer\GAL4^ppk23.2.695

(Sato et al., 2020)

Scer\GAL4^ppk23.2.695, robo1^GD2608 has abnormal neuroanatomy | adult stage | female phenotype, enhanceable by tei^RNAi.ex4.UAS/Scer\GAL4^ppk23.2.695