Expression of Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 disrupts wing growth.

Ubiquitous expression of Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PU results in lethality at both 25[o]C and 19[o]C.

Expression of Hsap\FUS^{Scer\UAS.T:Hsap\MYC} in neurons under the control of either Scer\GAL4^elav.PU or Scer\GAL4^D42 significantly reduces adult viability at 25[o]C (to ~60% and 40% respectively). The flies are fully viable at 19[o]C.

Expression of Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF results in dose-dependent eye degeneration in four day old flies, resulting in complete loss of ommatidial organisation in flies expressing two copies of Hsap\FUS^{Scer\UAS.T:Hsap\MYC}. The phenotype becomes progressively more severe with age.

The locomotor activity of male third instar larvae expressing Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 is markedly impaired.

The locomotor activity of two day old adult flies expressing Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^D42 is severely impaired. A similar effect is seen in 10 day old flies.

Third instar larvae expressing Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 show a substantial reduction in the area and the number of both large and small boutons in the neuromuscular junctions from ventral longitudinal muscles 6 and 7 of segment A2. The motor neurons in A1-A5 of the larval ventral nerve cord appear disorganised.

The nuclei of larval motor neurons in the ventral nerve cord of flies expressing Hsap\FUS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 have an abnormal structure. Reduced nuclear staining is seen, reminiscent of apoptotic bodies. In contrast to wild type, 20-30 TUNEL positive apoptotic cells are seen in the ventral nerve cord.