FB2024_03 , released June 25, 2024
Allele: Hsap\FUSΔ32.UAS.Tag:MYC
Open Close
General Information
Symbol
Hsap\FUSΔ32.UAS.Tag:MYC
Species
H. sapiens
Name
FlyBase ID
FBal0278302
Feature type
allele
Associated gene
Hsap\FUS
Associated Insertion(s)
Carried in Construct
P{UAS-FUS.Δ32.MYC}
Key Links
Transgenic product class
inframe_deletion
(Xia et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Xia et al., 2012)
Mutagen
in vitro construct
(Xia et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-FUS.Δ32.MYC}
Cytology
Description

UASt regulatory sequences drive expression of a mutant form of Hsap\FUS that truncates at amino acid 494. The construct is tagged with Tag:MYC.

(Xia et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\FUS
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The wings of flies expressing Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 appear wild type.

      Ubiquitous expression of Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act5C.PU reduces adult viability to approximately 80% at 25[o]C but complete viability is seen at 19[o]C.

      Four-day old flies expressing Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF have nearly wild type eye morphology and little, if any, retinal degeneration.

      The locomotor activity of male third instar larvae expressing Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4VGlut-OK371 is mildly impaired. A similar reduction in activity is seen in two day and ten day old adults when Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4D42.

      Third instar larvae expressing Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4VGlut-OK371 show little, if any, alteration in the number of either large and small boutons from ventral longitudinal muscles 6 and 7 of segment A2. The A1-A5 motor neurons appear organised and their nuclear structure appears normal. As in wild type, no apoptotic cells are detected in the ventral nerve cord.1G

      (Xia et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\FUSΔ32.Scer\UAS.T:Hsap\MYC
      Hsap\FUSΔ32.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)