FB2024_03 , released June 25, 2024
Allele: Avic\GFPCIETDY.UASp.Tag:Mito(CG9393)
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General Information
Symbol
Avic\GFPCIETDY.UASp.Tag:Mito(CG9393)
Species
A. victoria
Name
FlyBase ID
FBal0279419
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{UASp-CIETDY}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Mazzalupo and Cooley, 2006)
Progenitor genotype
Carried in construct
P{UASp-CIETDY}
Cytology
Description

UASp regulatory sequences drive expression of the CFP form of Avic\GFP, a linker sequence VIETDGA, the YFP form of Avic\GFP and the mitochondrial outer membrane targeting sequence from CG9393.

(Mazzalupo and Cooley, 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Avic\GFP
Tagged with
Tag:Mito(CG9393)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The linker between the CFP and YFP coding regions contains a IETD cleavage site that is recognised by the Dredd and Nc caspases. Upon cleavage by caspase, the CFP portion is no longer tethered to the mitochondrion-localised YFP and the YFP emission of ~530nm (mitochondrial) and CFP emission of ~480nm (cytoplasm) become spatially distinct.

      (Mazzalupo and Cooley, 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Avic\GFPCIETDY.Scer\UAS.P\T.T:Mito-CG9393
      Avic\GFPCIETDY.UASp.Tag:Mito(CG9393)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)