Amino acid replacement: K302R.
UAS regulatory sequences are cloned upstream of mutated p53 coding sequences.
partially lethal - majority die | pupal stage, with Scer\GAL4da.G32
partially lethal - majority die | third instar larval stage, with Scer\GAL4da.G32
Expression of p53K302R.Scer\UAS under the control of Scer\GAL4da.G32 results in early third instar larval lethality in approximately 55% of cases, with most of the rest dying as white pupa.