Animals expressing Sirt2dsRNA.Scer\UAS.cKa under the control of Scer\GAL4Act5C.PU may show pupal lethality, with the phenotype being more severe at 28[o]C than at 25[o]C. Many adults expressing Sirt2dsRNA.Scer\UAS.cKa under the control of Scer\GAL4Act5C.PU die just after eclosion and their survivorship decreases linearly.