FlyBase Allele Report: Dmel\Fife[ex1027]

General Information

Symbol

Dmel\Fife^ex1027

Species

D. melanogaster

Name

FlyBase ID

FBal0280546

Feature type

allele

Associated gene

Dmel\Fife

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

minos activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bruckner et al., 2012)

Mutagen

minos activity

(Bruckner et al., 2012)

Progenitor genotype

Mi{ET1}Fife^MB10889

(Bruckner et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 16.5kb deletion that removes all of the Fife gene except for the first 11 amino acid residues of isoform 3 and the splice donor site for isoforms 1 and 2. An ectopic splice event occurs in the mutants between exons 3 and 13 that can potentially be translated producing an ectopic isoform containing only the C2A and C2B domains.

(Bruckner et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Bruckner et al., 2012)

abnormal locomotor behavior | adult stage (with Df(3L)BSC412)

(Bruckner et al., 2012)

abnormal neuroanatomy | third instar larval stage

(Bruckner et al., 2012)

abnormal neuroanatomy | third instar larval stage (with Df(3L)BSC412)

(Bruckner et al., 2012)

abnormal neuroanatomy | third instar larval stage (with Df(3L)Exel6091)

(Bruckner et al., 2017)

abnormal neurophysiology | third instar larval stage

(Bruckner et al., 2017, Bruckner et al., 2012)

abnormal neurophysiology | third instar larval stage (with Df(3L)BSC412)

(Bruckner et al., 2012)

abnormal neurophysiology | third instar larval stage (with Df(3L)Exel6091)

(Bruckner et al., 2017)

abnormal neurophysiology | third instar larval stage (with Fife^AC)

(Bruckner et al., 2017)

female sterile

(Bruckner et al., 2012)

female sterile (with Df(3L)BSC412)

(Bruckner et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Fife^ex1027/Df(3L)Exel6091 transheterozygous third instar larvae exhibit defective neurotransmission across neuromuscular junctions, as assessed by decreased excitatory junction potential amplitude and decreased quantal content as compared to controls, and defective neuromuscular junction active zones, as shown by a significant decrease in the width of active zone T-bars, as compared to controls.

The neuromuscular junctions of Fife^ex1027 homozygous third instar larvae present an impaired synaptic probability of release compared to controls, as shown by the significant increase in failure to respond to presynaptic stimuli.

The neurotransmission across neuromuscular junctions of Fife^AC/Fife^ex1027 transheterozygous third instar larvae occurs with similar spontaneous miniature excitatory junction potentials, but decreased excitatory junction potentials and decreased quantal content, as compared to controls.

(Bruckner et al., 2017)

Synaptic area at the neuromuscular junction (NMJ) is normal in homozygous and Fife^ex1027/Df(3L)BSC412 third instar larvae. The number of active zones/NMJ and average active zone (AZ) length is also normal in these animals. However, a number of ultrastructural defects are seen in AZ architecture in Fife^ex1027/Df(3L)BSC412 third instar larvae. Detachments of the pre-synaptic membrane, or membrane ruffling, are seen in 91.7% of cases and those AZs with membrane defects are on average ruffled along 44% of their length. Non-membrane associated electron-dense structures of variable morphology that are capable of clustering synaptic vesicles away from AZs are seen in 12.1% of cases. There is an approximately 20% decrease in vesicle number at the mutant AZs compared to wild type, while total vesicle density is unaffected in the mutant boutons.

Frequency and amplitude of the miniature excitatory junction potentials (EJPs) at the neuromuscular junction are normal in Fife^ex1027/Df(3L)BSC412 third instar larvae. However, evoked EJPs and quantal content are significantly decreased compared to wild type by in homozygous and Fife^ex1027/Df(3L)BSC412 larvae.

Homozygous and Fife^ex1027/Df(3L)BSC412 females produce, but fail to lay, eggs.

Homozygous and Fife^ex1027/Df(3L)BSC412 adults show significantly reduced walking activity compared to controls.

(Bruckner et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Fife^ex1027/Df(3L)BSC412 is rescued by Scer\GAL4^elav-C155/Fife^UAS.Venus

(Bruckner et al., 2012)

Fife^ex1027/Df(3L)BSC412 is rescued by Scer\GAL4^elav-C155/Fife^UAS.cBa

(Bruckner et al., 2012)

Comments

The decreased evoked excitatory junctional potential and quantal content seen at the neuromuscular junction in Fife^ex1027/Df(3L)BSC412 third instar larvae and reduced walking activity of Fife^ex1027/Df(3L)BSC412 adults are completely rescued by expression of Fife^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155.

The reduced walking activity of Fife^ex1027/Df(3L)BSC412 adults are completely rescued by expression of Fife^{Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^elav-C155.

(Bruckner et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Fife^ex1027

(Bruckner et al., 2017)

Fife^ex

(Bruckner et al., 2017)

fife^EX1027

(Wentzel et al., 2018)

fife^ex1027

(Petzoldt et al., 2020, Bruckner et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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