FlyBase Allele Report: Dmel\rg[FDD]

General Information

Symbol

Dmel\rg^FDD

Species

D. melanogaster

Name

FlyBase ID

FBal0281961

Feature type

allele

Associated gene

Dmel\rg

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Volders et al., 2012)

Mutagen

FLPase

(Volders et al., 2012)

Progenitor genotype

PBac{WH}rg^f02898

(Volders et al., 2012)

PBac{WH}f02325

(Volders et al., 2012)

Cytology

Description

FLP-mediated recombination has deleted the genomic sequence between the two progenitor insertions, removing sequence extending from upstream of the second ATG codon of rg through the entire coding sequence and 3'UTR.

(Volders et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:5,205,115..5,282,581 [+]

Comment:

FLP-mediated recombination has deleted the genomic sequence between the two progenitor insertions, removing most of the coding sequence and all of the 3'UTR.

(Volders et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1^Δ50M

(Sears et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning

(Volders et al., 2012)

abnormal neuroanatomy | adult stage

(Volders et al., 2012)

abnormal neuroanatomy | adult stage (with Df(1)ovo41)

(Volders et al., 2012)

abnormal neuroanatomy | third instar larval stage

(Volders et al., 2012)

abnormal neuroanatomy | third instar larval stage (with Df(1)ovo41)

(Volders et al., 2012)

visible

(Volders et al., 2012)

Phenotype Manifest In

adult mushroom body

(Volders et al., 2012)

adult mushroom body (with Df(1)ovo41)

(Volders et al., 2012)

eye

(Volders et al., 2012)

NMJ bouton | third instar larval stage

(Volders et al., 2012)

NMJ bouton | third instar larval stage (with Df(1)ovo4)

(Volders et al., 2012)

Detailed Description

Statement

Reference

Mutants have a rough eye phenotype.

Mutant flies show a complete loss in aversive and appetitive memory when assayed immediately after olfactory conditioning.

68% of mushroom body hemispheres have morphological defects in hemizygous males and 77% of hemispheres have morphological defects in homozygous females. 56% of mushroom body hemispheres have morphological defects in rg^FDD/Df(1)ovo41 females.

Hemizygous male, homozygous female and rg^FDD/Df(1)ovo41 female third instar larvae have increased bouton numbers per muscle area at the neuromuscular junction compared to wild type.

Quantal content at the neuromuscular junction of mutant larvae is similar to wild type.

(Volders et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rg^FDD has visible phenotype, suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^GMR.PU

(Volders et al., 2012)

rg^FDD has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^nSyb.PS

(Volders et al., 2012)

NOT suppressed by

Statement

Reference

rg^FDD has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^FDD has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^FDD has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247

(Volders et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

rg^FDD has eye phenotype, suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^GMR.PU

(Volders et al., 2012)

rg^FDD has NMJ bouton | third instar larval stage phenotype, suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^nSyb.PS

(Volders et al., 2012)

NOT suppressed by

Statement

Reference

rg^FDD has adult mushroom body phenotype, non-suppressible by Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^FDD has adult mushroom body phenotype, non-suppressible by Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^FDD has adult mushroom body phenotype, non-suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247

(Volders et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PU rescues the rough eye phenotype of rg^FDD flies.

The mushroom body defects seen in hemizygous rg^FDD males are not rescued by expression of Mmus\Nbea^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU, Scer\GAL4^ey-OK107 or Scer\GAL4^Mef2.247.

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS rescues the increased bouton number at the neuromuscular junction which is seen in hemizygous rg^FDD male third instar larvae.

(Volders et al., 2012)

Complementation and Rescue Data

Rescued by

rg^FDD is rescued by rg^UAS.cVa/Scer\GAL4^GMR.PU

(Volders et al., 2012)

rg^FDD is rescued by rg^UAS.cVa/Scer\GAL4^nSyb.PS

(Volders et al., 2012)

Partially rescued by

rg^FDD is partially rescued by Scer\GAL4^elav.PU/rg^UAS.cVa

(Volders et al., 2012)

rg^FDD is partially rescued by Scer\GAL4^ey-OK107/rg^UAS.cVa

(Volders et al., 2012)

Not rescued by

rg^FDD is not rescued by Scer\GAL4^Mef2.247/rg^UAS.cVa

(Volders et al., 2012)

Comments

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PU rescues the rough eye phenotype of rg^FDD flies.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive memory seen in rg^FDD flies and partially rescues the loss of immediate appetitive memory seen in rg^FDD flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^FDD males are partially rescued by expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ey-OK107, but are not rescued by expression under the control of Scer\GAL4^Mef2.247.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS rescues the increased bouton number at the neuromuscular junction which is seen in hemizygous rg^FDD male third instar larvae.

(Volders et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

81125

rg^FDD P{neoFRT}19A/FM7a, P{sChFP}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rg^FDD

(Sears and Broadie, 2022, Sears et al., 2019, Volders et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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