FlyBase Allele Report: Mmus\Nbea[UAS.cVa]

General Information

Symbol

Mmus\Nbea^UAS.cVa

Species

M. musculus

Name

Saccharomyces cerevisiae UAS construct of Volders

FlyBase ID

FBal0281964

Feature type

allele

Associated gene

Mmus\Nbea

Associated Insertion(s)

Carried in Construct

P{UAS-Nbea.V}

Transgenic product class

cDNA

(Volders et al., 2012)

wild_type

(Volders et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Volders et al., 2012)

wild_type

(Volders et al., 2012)

Mutagen

in vitro construct

(Volders et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Nbea.V}

Cytology

Description

UASt regulatory sequences drive expression of a full-length Mmus\Nbea cDNA.

(Volders et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Nbea

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS has no effect on bouton number at the neuromuscular junction in third instar larvae.

(Volders et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mmus\Nbea^UAS.cVa/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of rg^FDD

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^nSyb.PS is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of rg^FDD

(Volders et al., 2012)

Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa is a suppressor | partially of abnormal learning phenotype of rg^γ5

(Volders et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^γ5

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^γ5

(Volders et al., 2012)

Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^FDD

(Volders et al., 2012)

Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^FDD

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^FDD

(Volders et al., 2012)

Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of rg^γ5

(Volders et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mmus\Nbea^UAS.cVa/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of rg^FDD

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^nSyb.PS is a suppressor of NMJ bouton | third instar larval stage phenotype of rg^FDD

(Volders et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa is a non-suppressor of adult mushroom body phenotype of rg^FDD

(Volders et al., 2012)

Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa is a non-suppressor of adult mushroom body phenotype of rg^FDD

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247 is a non-suppressor of adult mushroom body phenotype of rg^FDD

(Volders et al., 2012)

Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa is a non-suppressor of adult mushroom body phenotype of rg^γ5

(Volders et al., 2012)

Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa is a non-suppressor of adult mushroom body phenotype of rg^γ5

(Volders et al., 2012)

Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247 is a non-suppressor of adult mushroom body phenotype of rg^γ5

(Volders et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PU rescues the rough eye phenotype of rg^FDD flies.

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive memory seen in rg^γ5 flies but fails to rescue the loss of immediate appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^FDD and rg^γ5 males are not rescued by expression of Mmus\Nbea^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU, Scer\GAL4^ey-OK107 or Scer\GAL4^Mef2.247.

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS rescues the increased bouton number at the neuromuscular junction which is seen in hemizygous rg^FDD male third instar larvae.

(Volders et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78794

w^* rg^γ5; P{UAS-Nbea.V}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Nbea^Scer\UAS.cVa

Mmus\Nbea^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Volders

Secondary FlyBase IDs

References (1)

Report Sections

Open Close