FlyBase Allele Report: Dmel\mam[ΔC]

General Information

Symbol

Dmel\mam^ΔC

Species

D. melanogaster

Name

FlyBase ID

FBal0282352

Feature type

allele

Associated gene

Dmel\mam

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zhang et al., 2011)

Mutagen

ethyl methanesulfonate

(Zhang et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?959term.

(Zhang et al., 2011)

The mutation results in a protein that lacks the C-terminal acidic cluster and the three runs of glycine-valine residues.

(Zhang et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:14,055,871..14,055,871 [+]

Nucleotide change:

C14055871T

Amino acid change:

Q959term | mam-PA; Q959term | mam-PB; Q959term | mam-PD

Reported amino acid change:

?959term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zhang et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage 13

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16 (with mam⁸)

(Zhang et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

As in wild type, mam^ΔC mutant stage 13 embryos contain approximately 6 anterior midline glia (AMG), although these are more compact. However mam^ΔC mutants only have on average three posterior midline glia (PMG) compared to the 4 seen in wild type. During stages 14 and 15 the number of midline glia diminishes as in wild type, but by stage 16 mam^ΔC mutant embryos still contain around 5 AMG compared to only three in wild type. The extra AMGs are able to enwrap lateral axons. Both wild type and mam^ΔC mutant embryos show complete loss of PMGs by stage 16. Midline segmental compartments are less clearly defined than in wild type and, in many cases, glial processes extend into the posterior compartment. The nerve cord does not retract normally in mam^ΔC mutant embryos.

Midline glia are present in mam⁸/mam^ΔC mutant stage 16 embryos but appear disorganised in comparison with wild type.

Embryos that are maternally and zygotically mutant for mam^ΔC display gross developmental defects. The phenotype in embryos with either no zygotic copies of mam or one zygotic copy are indistinguishable.

As in wild type, mam^ΔC mutant embryos contain two MP1 neurons per segment. The H cell sib is absent and the number of H cells is increased to six, compared to only one in wild type.

mam^ΔC mutant stage 16 embryos contain 11 mVUM cells per segment, compared to three in wild type.

The iVUM and the progeny of the MNB appear to be absent in mam^ΔC mutant stage 16 embryos.

All midline cells within mam^ΔC mutant embryos remain at the dorsal side of the nerve cord.

(Zhang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, mam^ΔC has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT Enhanced by

Statement

Reference

mam^ΔC has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by spi^s.UAS/Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

mam^ΔC has abnormal neuroanatomy | embryonic stage 16 phenotype, non-suppressible by spi^s.UAS/Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

mam^ΔC is a non-enhancer of abnormal neuroanatomy | embryonic stage 13 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

mam^ΔC is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

NOT Suppressor of

Statement

Reference

mam^ΔC is a non-suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

mam^ΔC is a non-suppressor of abnormal neuroanatomy | embryonic stage 13 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

mam^ΔC is a non-suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

mam^ΔC has midline glial cell | embryonic stage 16 | increased number phenotype, enhanceable by Scer\GAL4^sim.P3.7/N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

NOT Enhanced by

Statement

Reference

mam^ΔC has midline glial cell | embryonic stage 16 | increased number phenotype, non-enhanceable by spi^s.UAS/Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

mam^ΔC has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by spi^s.UAS/Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

mam^ΔC is a non-enhancer of midline glial cell | embryonic stage 13 | increased number phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

mam^ΔC is a non-enhancer of midline glial cell | embryonic stage 16 | increased number phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

NOT Suppressor of

Statement

Reference

mam^ΔC is a non-suppressor of midline glial cell | increased number | embryonic stage 16 phenotype of N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

mam^ΔC is a non-suppressor of midline glial cell | increased number | embryonic stage 13 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

mam^ΔC is a non-suppressor of midline glial cell | increased number | embryonic stage 16 phenotype of Scer\GAL4^sim.P3.7, spi^s.UAS

(Zhang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

mam^ΔC does not suppress the extra anterior midline cells seen in stage 13 embryos when spi^s.Scer\UAS is expressed under the control of Scer\GAL4^sim.P3.7. At stage 16, embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^sim.P3.7 in a mam^ΔC mutant background contain a similar number of extra anterior midline glia cells as in either mutant alone.

Expression of N^{Intra1768.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^sim.P3.7 in a mam^ΔC mutant background enhances the increase in anterior midline glial cells seen in mam^ΔC mutant stage 16 embryos, producing a phenotype similar to that seen when N^{Intra1768.Scer\UAS.T:Zzzz\FLAG} is expressed alone.

(Zhang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mam^ΔC

(Zhang et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close