Expression of Hr4dsRNA.Scer\UAS in the ring gland, under the control of Scer\GAL4P0206 triggers phenotypes consistent with early larval wandering and results in early pupariation and small precocious prepupae.
Expression of Hr4dsRNA.Scer\UAS in the fat body, under the control of Scer\GAL4Cg.PA results in prepupal lethality in approximately 10% of the population. There is also a failure to evert anterior spiracles, incomplete or absent head eversion, and incorrect location of the gas bubble in these flies, indicative of a defect in ecdysone hierarchy. These flies do not exhibit any timing defects or abnormally sized pupae.