FB2024_03 , released June 25, 2024
Allele: Dmel\mysN840A.EYFP.Ubi-p63E
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General Information
Symbol
Dmel\mysN840A.EYFP.Ubi-p63E
Species
D. melanogaster
Name
FlyBase ID
FBal0282390
Feature type
allele
Associated gene
Dmel\mys
Associated Insertion(s)
Carried in Construct
P{Ubi-mys-YFP.N840A}
Key Links
Transgenic product class
missense_variant
(Pines et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Pines et al., 2011)
Mutagen
in vitro construct
(Pines et al., 2011)
Progenitor genotype
Carried in construct
P{Ubi-mys-YFP.N840A}
Cytology
Description

Ubi-p63E promoter sequences drive expression of mys coding sequences carrying a predicted deactivating mutation in the distal NPxY motif of the cytoplasmic domain (carries the amino acid replacement N840A, predicted to disrupt the binding of a subset of signaling molecules that are required for inside-out integrin activation).

(Pines et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
mys
Tagged with
EYFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      mysN840A.EYFP.Ubi-p63E partially rescues mys11

      (Pines et al., 2011)
      Comments

      Expression of mysN840A.T:Avic\GFP-EYFP.Ubi-p63E partially rescues both the germband retraction and dorsal closure phenotypes found in mys11 embryos.

      The mysN840A.T:Avic\GFP-EYFP.Ubi-p63E transgene partially rescues the muscle detachment phenotype seen in mys11 embryos. Although myotendinous junction length appears fully rescued in these animals, there is a mild but significant reduction in myotendinous area compared to wild-type.

      (Pines et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mysN840A.EYFP.Ubi-p63E
      mysN840A.T:Avic\GFP-EYFP.Ubi-p63E
      Name Synonyms
      Secondary FlyBase IDs
        References (3)