FB2024_03 , released June 25, 2024
Allele: Scer\GAL4ple.TH-C
Open Close
General Information
Symbol
Scer\GAL4ple.TH-C
Species
S. cerevisiae
Name
FlyBase ID
FBal0282520
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{ple-GAL4.TH-C}
Also Known As
TH-C1-Gal4, TH-C-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Liu et al., 2012)
Progenitor genotype
Carried in construct
P{ple-GAL4.TH-C}
Cytology
Description

A sub-fragment of the genomic ple sequence present in Scer\GAL4ple.PF is fused upstream of the GAL4 coding region.

(Liu et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
ple_TH-C
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval dopaminergic DL2 neuron
      (Bjordal et al., 2014)
      larval dopaminergic DL1 neuron | posterior | subset
      (Bjordal et al., 2014)
      larval dopaminergic DM1 neuron
      (Bjordal et al., 2014)
      (Bjordal et al., 2014)
      adult stage
      dopaminergic PAM neuron | subset
      (Agrawal and Hasan, 2015, Liu et al., 2012)
      dopaminergic PAL neuron | subset
      (Xie et al., 2018, Liu et al., 2012)
      dopaminergic PPM1 neuron
      (Agrawal and Hasan, 2015, Liu et al., 2012)
      dopaminergic PPM2 neuron | subset
      (Xie et al., 2018, Liu et al., 2012)
      dopaminergic PPL2ab neuron
      (Xie et al., 2018, Agrawal and Hasan, 2015, Liu et al., 2012)
      dopaminergic PAL neuron
      (Agrawal and Hasan, 2015)
      dopaminergic PPM2 neuron
      (Agrawal and Hasan, 2015)
      dopaminergic PPL1 neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of prothoracic neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of mesothoracic neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of metathoracic neuron
      (Agrawal and Hasan, 2015)
      dopaminergic neuron of abdominal neuron
      (Agrawal and Hasan, 2015)
      dopaminergic PPM1 neuron | subset
      (Xie et al., 2018)
      adult dopaminergic neuron of gnathal ganglion | subset
      (Xie et al., 2018)
      adult lateral accessory lobe-posterior slope-protocerebral bridge neuron
      (Xie et al., 2018)
      Additional Information
      Statement
      Reference

      ScerGAL4ple.TH-C drives expression in 6-49 PAM and PPM1 dopaminergic neurons, and 5 or fewer dopaminergic neurons in each other cluster where it is expressed.

      (Agrawal and Hasan, 2015)

      ScerGAL4ple.TH-C drives expression three anterior cells of the larval dopaminergic DL1 neuron cluster.

      (Bjordal et al., 2014)

      ScerGAL4ple.TH-C drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ScerGAL4ple.PF : around 10 PAM neurons, 2 PAL neurons, 1 PPM1 neuron, 6 PPM2 neurons and 6 PPL2 neurons. There was also some non-systematic expression in less than 1 neuron in the PPL1 cluster.

      (Liu et al., 2012)
       
      Marker for
      Reflects expression of
      ple
      (Xie et al., 2018, Bjordal et al., 2014, Liu et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4ple.TH-C cells in adults does not affect startle-induced climbing behavior, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4ple.TH-C cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

      (Sun et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4ple.TH-C
      Name Synonyms
      Secondary FlyBase IDs
        References (13)