FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Rab27.ORF
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General Information
Symbol
Scer\GAL4Rab27.ORF
Species
S. cerevisiae
Name
FlyBase ID
FBal0283191
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
PBac{I-SceI(FRT.Rab27-GAL4.ORF(loxP.3xP3-RFP))}
Also Known As
rab27-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Chan et al., 2011)
Progenitor genotype
Carried in construct
PBac{I-SceI(FRT.Rab27-GAL4.ORF(loxP.3xP3-RFP))}
Cytology
Description

Contains a genomic fragment centred on Rab27 in which the complete Rab27 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.

(Chan et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Rab27
Encoded product / tool
GAL4
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      neuron | subset of embryonic/larval brain
      (Chan et al., 2011)
      glial cell | subset of embryonic/larval brain
      (Chan et al., 2011)
      neuron | subset of larval ventral nerve cord
      (Chan et al., 2011)
      Bolwig organ | restricted
      (Jin et al., 2012)
      embryonic/larval mushroom body | intense
      (Jin et al., 2012)
      leg disc | restricted
      (Jin et al., 2012)
      pupal stage P6 -- P7
      neuron | subset of pupal brain

      Comment: 30-40h APF

      (Chan et al., 2011)
      mushroom body

      Comment: 30-40h APF

      (Chan et al., 2011)
      mushroom body | intense

      Comment: 30-40h APF

      (Jin et al., 2012)
      eye photoreceptor cell

      Comment: 30-40h APF

      (Jin et al., 2012)
      adult stage
      neuron | subset of adult brain
      (Chan et al., 2011)
      adult mushroom body | intense
      (Chan et al., 2011)
      adult mushroom body
      (Jin et al., 2012)
      neuron of lamina | faint
      (Jin et al., 2012)
      Additional Information
      Statement
      Reference

      ScerGAL4Rab27.ORF drives expression in many neurons and possibly glia in the larval brain. In the pupal and adult, expression in the brain is restricted to a few central neurons, including the mushroom bodies. Expression in the adult mushroom body is stronger than in the pupal one.

      (Chan et al., 2011)
       
      Marker for
      Reflects expression of
      Rab27
      (Jin et al., 2012, Chan et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The PBac{I-SceI(FRT.Rab27-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab27 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab27 locus.

      (Chan et al., 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Rab27.ORF
      Name Synonyms
      Secondary FlyBase IDs
        References (4)