Contains a genomic fragment centred on Rab7 in which the complete Rab7 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.
ScerGAL4Rab7.ORF drives expression in some neurons and glia in the larval brain, as well as in the developing photoreceptors and in regions of the wing imaginal disc. In the pupal and adult brain, expression is widespread. Expression in the adult mushroom body is stronger than in the pupa.
The PBac{I-SceI(FRT.Rab7-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab7 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab7 locus.