FlyBase Allele Report: Dmel\Cog7[z-4495]

General Information

Symbol

Dmel\Cog7^z-4495

Species

D. melanogaster

Name

FlyBase ID

FBal0284626

Feature type

allele

Associated gene

Dmel\Cog7

Associated Insertion(s)

Carried in Construct

Also Known As

Cog7^z4495

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: ??term.

(Belloni et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:29,932,576..29,932,576 [-]

Nucleotide change:

G29932576A

Amino acid change:

W134term | Cog7-PA

Reported amino acid change:

W134term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Belloni et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of congenital disorder of glycosylation type IIe

CEA with Cog7^z-5797

(Frappaolo et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male (with Df(3R)BSC861)

(Belloni et al., 2012)

abnormal cytokinesis | spermatogenesis (with Cog7^z-5797)

(Frappaolo et al., 2017)

abnormal locomotor behavior | adult stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

abnormal locomotor behavior | larval stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

abnormal neuroanatomy | larval stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

male sterile

(Belloni et al., 2012)

male sterile (with Cog7^z-2805)

(Belloni et al., 2012)

male sterile (with Cog7^z-5797)

(Belloni et al., 2012)

paralytic | adult stage | heat sensitive (with Cog7^z-5797)

(Frappaolo et al., 2017)

short lived (with Cog7^z-5797)

(Frappaolo et al., 2017)

viable

(Belloni et al., 2012)

viable (with Cog7^z-2805)

(Belloni et al., 2012)

viable (with Cog7^z-5797)

(Frappaolo et al., 2017, Belloni et al., 2012)

visible | adult stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

Phenotype Manifest In

contractile ring (with Df(3R)BSC861)

(Belloni et al., 2012)

embryonic/larval neuromuscular junction | larval stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

NMJ bouton | larval stage (with Cog7^z-5797)

(Frappaolo et al., 2017)

nucleus | spermatogenesis (with Cog7^z-5797)

(Frappaolo et al., 2017)

spermatid

(Belloni et al., 2012)

spermatid (with Cog7^z-2805)

(Belloni et al., 2012)

spermatid (with Cog7^z-5797)

(Frappaolo et al., 2017, Belloni et al., 2012)

spermatid (with Df(3R)BSC861)

(Belloni et al., 2012)

wing (with Cog7^z-5797)

(Frappaolo et al., 2017)

Detailed Description

Statement

Reference

Cog7^z-4495/Cog7^z-5797 transheterozygotes exhibit pronounced locomotor defects: larvae show irregular crawling with more turns and shorter distances, decreased frequency of peristaltic contractions and decreased distance per contraction, as compared to controls; adults show decreased climbing ability, as compared to controls. These individuals also show a held-out wing phenotype and present heat sensitive paralysis (38[o]C). About half spermatids in testes are multinucleate, indicating cytokinesis failure.

Cog7^z-4495/Cog7^z-5797 transheterozygous larvae show exhibit neuromuscular junctions with significantly fewer boutons, but similar primary branch number, as compared to controls.

(Frappaolo et al., 2017)

The majority of spermatids are multinucleate, suggesting a failure of meiotic cytokinesis, in homozygous, Cog7^z-4495/Cog7^z-5797, Cog7^z-4495/Cog7^z-2805 and Cog7^z-4495/Df(3R)BSC861 males.

Cog7^z-4495/Cog7^z-5797 males show a failure of spermatid elongation.

The F-actin rings fail to constrict to completion and the central spindles appear less dense than wild type in 80% of mid-late telophase I figures in Cog7^z-4495/Df(3R)BSC861 spermatocytes.

(Belloni et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cog7^z-4495/Cog7^z-5797 has paralytic | adult stage | heat sensitive phenotype, enhanceable by SiaT[+]/SiaT^L22

(Frappaolo et al., 2017)

Cog7^z-4495/Cog7^z-5797 has paralytic | adult stage | heat sensitive phenotype, enhanceable by sau^Z2217/sau[+]

(Frappaolo et al., 2017)

Suppressed by

Statement

Reference

Cog7^z-4495/Cog7^z-5797 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^Act5C.PU/Rab1^UAS.YFP

(Frappaolo et al., 2017)

Cog7^z-4495/Cog7^z-5797 has paralytic | adult stage | heat sensitive phenotype, suppressible by Scer\GAL4^Act5C.PU/Rab1^UAS.YFP

(Frappaolo et al., 2017)

Other

Statement

Reference

Cog7[+]/Cog7^z-4495, sau^Z2217 has visible | adult stage phenotype

(Frappaolo et al., 2017)

Cog7^z-4495/Cog7^z-5797, sau^Z2217 has lethal phenotype

(Frappaolo et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cog7^z-4495/Cog7^z-5797 has spermatid phenotype, suppressible by Scer\GAL4^Act5C.PU/Rab1^UAS.YFP

(Frappaolo et al., 2017)

Cog7^z-4495/Cog7^z-5797 has nucleus | spermatogenesis phenotype, suppressible by Scer\GAL4^Act5C.PU/Rab1^UAS.YFP

(Frappaolo et al., 2017)

Enhancer of

Statement

Reference

Cog7[+]/Cog7^z-4495 is an enhancer of spermatid phenotype of sau^Z2217/Df(2L)Exel7010

(Frappaolo et al., 2017)

Cog7[+]/Cog7^z-4495 is an enhancer of nucleus | spermatogenesis phenotype of sau^Z2217/Df(2L)Exel7010

(Frappaolo et al., 2017)

Other

Statement

Reference

Cog7[+]/Cog7^z-4495, sau^Z2217 has wing phenotype

(Frappaolo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

sau^Z2217/sau^Z2217, Cog7^z-4495/+ adults exhibit held-out wings, which also appear swollen and crumpled.

(Frappaolo et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cog7^z-4495

(Belloni et al., 2012)

Cog7^z4495

(Frappaolo et al., 2017, Sechi et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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