Imprecise excision of the progenitor insertion, resulting in a deletion that removes approximately 500bp from exon 2 and intron 2-3 of Socs36E. This introduces a frame-shift and generates an in-frame TAA stop codon which produces a truncated protein of 178 amino acid residues.
Approximate boundaries of a ~500 bp deletion resulting from the imprecise excision of P{EPgy2}Socs36EEY06665. The boundary within the first coding exon is from sequence data. The boundary in the intron is estimated. The predicted protein product has 175 aa of Socs36E followed by 3 novel amino acids before termination.
Abnormally invasive cells trail behind the main border cell cluster and are detached from it in 63% of mutant stage 10 egg chambers.
Socs36E[+]/Socs36E178, mir-279ex117 has border follicle cell phenotype
Socs36E[+]/Socs36E178, mir-279ex36 has border follicle cell phenotype
Socs36E178, apt167/apt[+] has border follicle cell phenotype
Egg chambers of Socs36E178/+, apt167/+ double heterozygous females contain additional invasive cells that are not associated with the border cell cluster: 55% of the double heterozygous egg chambers contain more than one additional invasive cell compared to 10% and 15% respectively for each single heterozygote.
In 44% of Socs36E178 aptKG05830/Socs36EEY06665 apt167 stage 10 egg chambers the border cells are tethered to the anterior end of the egg chamber by non-cluster associated invasive cells. This inability to detach from neighbouring follicle cells results in a migration delay in 31% of the stage 10 double mutant egg chambers.
Egg chambers of Socs36E178/+, mir-279Δ1.2/+ and of Socs36E178/+, mir-279Δ1.9/+ double heterozygous females contain additional invasive cells that are not associated with the border cell cluster compared to either single heterozygote.
