FB2024_03 , released June 25, 2024
Allele: Dmel\AmnionlessVDRC.cUa
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General Information
Symbol
Dmel\AmnionlessVDRC.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0287548
Feature type
allele
Associated gene
Dmel\Amnionless
Associated Insertion(s)
Carried in Construct
P{VDRC.Amnionless.U}
Key Links
Transgenic product class
RNAi_reagent
(Zhang et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Zhang et al., 2013)
Mutagen
in vitro construct
(Zhang et al., 2013)
Progenitor genotype
Carried in construct
P{VDRC.Amnionless.U}
Cytology
Description

UAS regulatory sequences drive expression of a Amnionless RNAi construct.

(Zhang et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Amnionless
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Uptake of a secreted marker protein by pericardial nephrocytes is blocked in animals expressing AmnionlessVDRC.cUa under the control of Scer\GAL4Dot.PK. The pericardial nephrocytes show ultrastructural defects compared to wild type: the number of endocytic vesicles and vacuoles are dramatically reduced, electron-dense lysosomes are nearly absent, nephrocyte diaphragms are reduced in number and lacunar structures are shortened.

      Animals expressing AmnionlessVDRC.cUa under the control of Scer\GAL4Dot.PK are viable under normal conditions, but show dramatically reduced viability compared to controls in the presence of silver nitrate.

      (Zhang et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The block in uptake of a secreted marker protein by pericardial nephrocytes caused by expression of AmnionlessVDRC.cUa under the control of Scer\GAL4Dot.PK is rescued by co-expression of Hsap\AMNScer\UAS.cZa.

      (Zhang et al., 2013)
      Complementation and Rescue Data
      Rescued by

      AmnionlessVDRC.cUa is rescued by AmnionlessUAS.cZa/Scer\GAL4Ugt36A1.PK

      (Zhang et al., 2013)
      Comments

      The block in uptake of a secreted marker protein by pericardial nephrocytes caused by expression of AmnionlessVDRC.cUa under the control of Scer\GAL4Dot.PK is rescued by co-expression of AmnionlessScer\UAS.cZa.

      (Zhang et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      AmnionlessVDRC.cUa
      Name Synonyms
      Secondary FlyBase IDs
        References (1)