21.8% of pupae expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act5C.PU (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) hatch successfully at 25[o]C. No adults hatch at 29[o]C.

Mitochondrial ultrastructure is abnormal in larvae expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act5C.PU (in the presence of Dcr-2^Scer\UAS.cDa) at 29[o]C. The matrices are swollen and electron pale, there is a loss of mitochondrial cristae and the mitochondria are significantly enlarged.

Lactate concentration is increased compared to wild type in larvae expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act5C.PU (in the presence of Dcr-2^Scer\UAS.cDa). Glycogen concentration is decreased.

Animals expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^nub-AC-62 (in the presence of Dcr-2^Scer\UAS.cDa) show tissue damage in the wing, with a penetrance of 32.3% at 25[o]C and 97.7% at 29[o]C. Relative mtDNA copy number is increased compared to wild type.

Partial or total loss of the anterior crossvein is seen in 4% of flies expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^ptc-559.1 (in the presence of Dcr-2^Scer\UAS.cDa) at 29[o]C. The L3-L4 intervein region is significantly reduced in area compared to wild type, with the reduction being due to a decrease in cell number and not in cell size.

Flies expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^repo.PU (in the presence of Dcr-2^Scer\UAS.cDa) have an altered survival curve compared to controls, with a reduction in half-life from 32 to 21 days at 29[o]C.

Adults expressing Aats-ser^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Mef2.PU (in the presence of Dcr-2^Scer\UAS.cDa) have reduced climbing ability compared to controls.