A 3146 base pair fragment from the flanking non-coding or intronic region of rut is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Thermogenetic activation (using TrpA1UAS.(B).cKa at 31.5[o]C) of Ecol\lexAGMR14H06 cells in adult males significantly reduces the amount of sleep, compared to controls, and results in extra rebound sleep after activation; activation does not significantly affect locomotor activity of awake adult males, compared to controls.
Ecol\lexAGMR14H06, TrpA113xlexAop.JFRC26 has abnormal sleep | adult stage | heat sensitive phenotype, enhanceable | heat sensitive by Scer\GAL4DBD.R19F09/shits1.IVS.p10.20xUAS/Hsap\RELAAD.R25D01
Ecol\lexAGMR14H06, TrpA113xlexAop.JFRC26 has abnormal sleep | adult stage | heat sensitive phenotype, suppressible | heat sensitive by Scer\GAL4DBD.R15B01/Hsap\RELAAD.R14C08/shits1.IVS.p10.20xUAS
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.