UAS regulatory sequences drive expression of a chimeric protein composed of Gli fused to the C-terminus of the extracellular domain of spi, replacing the palmitoylation site. The construct is tagged with a Tag:HA epitope.
eye disc | somatic clone, with Scer\GAL4Act5C.PU
photoreceptor | ectopic | somatic clone, with Scer\GAL4Act5C.PU
Somatic clones in eye imaginal discs that express Gli::spiCS.s.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PU recruit ectopic photoreceptors.