UAS regulatory sequences drive expression of a short inverted repeat.
Expression of IRSp53HMC02920 under the control of Scer\GAL4kirre.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in defective splitting of indirect flight muscle fibers, and fusion defects in myotubes, with a reduction in the number of small nuclei originating from fusing myoblasts.
IRSp53HMC02920, Scer\GAL4kirre.PU is an enhancer of abnormal cell shape | P-stage phenotype of Scer\GAL4kirre.PU, enaGD8910
IRSp53HMC02920, Scer\GAL4kirre.PU is an enhancer of myotube | P-stage phenotype of Scer\GAL4kirre.PU, enaGD8910
IRSp53HMC02920, Scer\GAL4kirre.PU is an enhancer of filopodium | P-stage phenotype of Scer\GAL4kirre.PU, enaGD8910
Co-expression of IRSp53HMC02920 does not significantly enhance the effect on myotube filopodia length, but does result in a significant reduction in filopodia density, and an increase in severity of subsequent muscle fiber splitting in the indirect flight muscle of flies expressing enaGD8910 under the control of Scer\GAL4kirre.PU.