FlyBase Allele Report: Dmel\nub[UAS.ORF.GW.Tag:HA]

UAS regulatory sequences drive expression of a full-length nub open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Also carries

recombinase target site

FRT5

recombinase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The T1 and T2 segments of stage 16 embryos expressing nub^{Scer\UAS.ORF.GW.T:Ivir\HA1} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^lbe.K show significant increases in the numbers of dividing neuroblasts and dividing neuroblast daughters, as compared to controls.

(Bahrampour et al., 2017)

Expression of nub^{Scer\UAS.ORF.GW.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 results in female flies with severe wing size and patterning defects at 18[o]C.

(Schertel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Phenotype Manifest In

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

The triple co-expression of hb^UAS.Tag:MYC, Kr^UAS.Tag:V5 and nub^{UAS.ORF.GW.Tag:HA} under the control of Scer\GAL4^da.G32 triggers additional neuroblast and extensive over-proliferation in stage 12 embryos, as compared to controls.

The sextuple co-expression of ase^UAS.Tag:MYC, wor^UAS.Tag:FLAG, SoxN^UAS.Tag:V5, Kr^UAS.Tag:V5, hb^UAS.Tag:MYC and nub^{UAS.ORF.GW.Tag:HA}, under the control of Scer\GAL4^da.G32 triggers the striking generation of ectopic neuroblasts throughout the ectoderm of stage 12 embryos, with extensive ectopic proliferation, as compared to controls.