FB2024_03 , released June 25, 2024
Allele: Dmel\UQCR-14KK115522
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General Information
Symbol
Dmel\UQCR-14KK115522
Species
D. melanogaster
Name
FlyBase ID
FBal0298809
Feature type
allele
Associated gene
Dmel\UQCR-14
Associated Insertion(s)
Carried in Construct
P{KK115522}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Homem et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Homem et al., 2014)
Mutagen
in vitro construct
(Homem et al., 2014)
Progenitor genotype
Carried in construct
P{KK115522}
Cytology
Description

UASt regulates expression of dsRNA that targets UQCR-14 (and CG32576) for RNAi. (UQCR-14 lies in an intronic region of CG32576.)

(Homem et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK115522
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent proliferation of larval neuroblasts (NBs), and increases the size and extends the lifespan of pupal NBs.

      Cultured pupal neuroblasts (NBs) from flies expressing UQCR-14KK115522 under the control of Scer\GAL4wor.PA and Scer\GAL80ase.PN are able to divide, but the normal shrinkage observed in wild type pupal NBs is completely prevented.

      Expression via Scer\GAL4insc-Mz1407 reduces oxygen consumption rates in larval and pupal brains. NADH levels are significantly increased, but overall energy/ATP levels are unchanged. Lactate levels are reduced and unchanged between larval and pupal stages, whereas lactate levels strongly decrease from larval to pupal stages in wild type.

      (Homem et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      UQCR-14KK115522
      Name Synonyms
      Secondary FlyBase IDs
        References (2)