Gene silencing using Scer\GAL4Act.PU>Rack1dsRNA.Scer\UAS.cUa is embryonic lethal.
Conditional expression of Rack1dsRNA.Scer\UAS.cUa only in adult flies using Scer\GAL80ts.αTub84B and Scer\GAL4Act.PU results in normal development. The adult onset expression of Rack1dsRNA.Scer\UAS.cUa does not decrease the number of animals emerging as adults, however, it reduces longevity by 20%. When these flies are challenged with Drosophila C virus (DCV), viral replication is markedly reduced compared with controls.