A genomic construct containing the entire coding region of α-PheRS and ~1kb of upstream and downstream sequences, and containing an A456G substitution that is predicted to increase the activation error rate of the enzyme.
C8144129G
A456G | alpha-PheRS-PA
A456G
Carried on a transgenic construct. Predicted to increase the activation error rate of the enzyme. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
α-PheRSA456G fails to rescue α-PheRSG2060
α-PheRSA456G does not rescue the lethality of α-PheRSG2060.