FlyBase Allele Report: Dmel\mir-1000[KO1]

General Information

Symbol

Dmel\mir-1000^KO1

Species

D. melanogaster

Name

FlyBase ID

FBal0301888

Feature type

allele

Associated gene

Dmel\mir-1000

Associated Insertion(s)

TI{TI}mir-1000^KO1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Verma et al., 2015)

Mutagen

ends-out gene targeting

(Verma et al., 2015, Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

cre recombinase

(Verma et al., 2015, Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-1000^KO1

Cytology

Description

141bp spanning the mir-1000 hairpin has been deleted. A single loxP site is present at the site of the deletion. Expression of the msi gene (mir-1000 is located within an intron of msi) is unaffected.

(Verma et al., 2015)

The mir-1000 hairpin has been deleted. A single loxP site is present at the site of the deletion. The expression of the msi host gene appears unaffected (assayed by quantitative PCR).

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA with mir-1000^KO2

(Verma et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive (with mir-1000^KO2)

(Verma et al., 2015)

abnormal neuroanatomy | adult stage (with mir-1000^KO2)

(Verma et al., 2015)

abnormal neuroanatomy | larval stage (with mir-1000^KO2-GAL4)

(Verma et al., 2015)

abnormal neurophysiology | larval stage

(Verma et al., 2015)

abnormal oxidative stress response | adult stage (with mir-1000^KO2)

(Verma et al., 2015)

female sterile (with Df(3R)Exel6203)

(Chen et al., 2014)

lethal | recessive

(Chen et al., 2014)

male semi-sterile (with Df(3R)Exel6203)

(Chen et al., 2014)

partially lethal (with Df(3R)Exel6203)

(Verma et al., 2015, Chen et al., 2014)

partially lethal (with mir-1000^KO2)

(Verma et al., 2015)

short lived (with Df(3R)Exel6203)

(Verma et al., 2015)

short lived (with mir-1000^KO2)

(Verma et al., 2015)

short lived | female (with Df(3R)Exel6203)

(Chen et al., 2014)

short lived | male (with Df(3R)Exel6203)

(Chen et al., 2014)

some die during embryonic stage (with Df(3R)Exel6203)

(Verma et al., 2015)

some die during embryonic stage (with mir-1000^KO2)

(Verma et al., 2015)

Phenotype Manifest In

NMJ bouton | larval stage (with mir-1000^KO2-GAL4)

(Verma et al., 2015)

Detailed Description

Statement

Reference

mir-1000^KO1/mir-1000^KO2 and mir-1000^KO2/Df(3R)Exel6203 adults show a sharply reduced lifespan, with survival declining rapidly by 2 weeks of age. mir-1000^KO1/mir-1000^KO2 adults show a progressive decline in climbing ability, with significant defects already seen at 2 days of age. Caspase-3 positive cells are seen in the brains of 2 and 10 day old mir-1000^KO1/mir-1000^KO2 adults and vacuoles are seen in 10 day old brains.

mir-1000^KO1/mir-1000^KO2 adults show reduced survival on exposure to hydrogen peroxide compared to controls.

The number and size of synaptic boutons at the neuromuscular junction is increased compared to controls in mir-1000^KO1/mir-1000^KO2-GAL4 larvae.

Homozygous larvae show a significant increase in the amplitude of spontaneous excitatory junction potentials at the neuromuscular junction.

(Verma et al., 2015)

mir-1000^KO/Df(3R)Exel6203 males and females show a significant decrease in median lifespan compared to controls.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible | partially by Scer\GAL4^NP6701/Diap1^UAS.cUa

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by mGluR^MI02169/mGluR[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by Nmdar1^EP331/Nmdar1[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by Scer\GAL4^NP6701/Diap1^UAS.cUa

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible | partially by Df(2L)BSC37/+

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible | partially by Nmdar1^EP331/Nmdar1[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible | partially by GluRIA^f05411/GluRIA[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible by mGluR^MI02169/mGluR[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by Df(2L)BSC37/+

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by GluRIA^f05411/GluRIA[+]

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, suppressible by mGluR^GD707/Scer\GAL4^NP6701

(Verma et al., 2015)

mir-1000^KO2/mir-1000^KO1 has short lived phenotype, suppressible by mGluR^GD707/Scer\GAL4^NP6701

(Verma et al., 2015)

NOT suppressed by

Statement

Reference

mir-1000^KO2/mir-1000^KO1 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Df(2L)Exel8016/+

(Verma et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^Scer\UAS.cUa under the control of Scer\GAL4^mGluR-NP6701 partially suppresses the short lived phenotype of mir-1000^KO1/mir-1000^KO2 adults and reduces the number of Caspase-3 positive cells seen in the adult brain of these flies. The climbing defects are also rescued.

The climbing defects of two day old mir-1000^KO1/mir-1000^KO2 adults are rescued if they are also carrying Df(2L)BSC37/+. The amount of Caspase-3 positive cells in the brain is reduced, and lifespan is increased (although not to control levels).

The climbing defects of mir-1000^KO1/mir-1000^KO2 adults are rescued if they are also carrying Nmdar1^EP331/+, and lifespan is increased (although not to control levels).

The climbing defects of mir-1000^KO1/mir-1000^KO2 adults are rescued if they are also carrying GluRIA^f05411/+, and lifespan is increased (although not to control levels).

The climbing defects and shortened lifespan of mir-1000^KO1/mir-1000^KO2 adults are rescued if they are also carrying mGluR^MI02169/+.

The climbing defects and shortened lifespan of mir-1000^KO1/mir-1000^KO2 adults are rescued if they are also carrying mGluR^GD707.

The climbing defects of mir-1000^KO1/mir-1000^KO2 adults are not rescued by Df(2L)Exel8016/+.

(Verma et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58882

w^*; TI{TI}mir-1000^KO1/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

miR-1000^KO1

(Verma et al., 2015)

mir-1000^KO1

mir-1000^KO

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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