FB2024_03 , released June 25, 2024
Allele: Ecol\lexAVP16.Gr5a
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General Information
Symbol
Ecol\lexAVP16.Gr5a
Species
E. coli
Name
construct a of Fujii
FlyBase ID
FBal0304292
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
TI{lexA::VP16}Gr5aLEXA
Carried in Construct
Also Known As
Gr5aLexA, Gr5a-lexA
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Fujii et al., 2015)
Progenitor genotype
Associated Insertion(s)
TI{lexA::VP16}Gr5aLEXA
Cytology
Description

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver.

(Fujii et al., 2015)

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver. A single loxP site is also present downstream of the inserted driver sequence.

(Yavuz et al., 2014)
Allele components
Component
Use(s)
Inserted element
TI{lexA::VP16}
Encoded product / tool
lexA::VP16
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      water-sensing neuron of labellar taste bristle of labellar taste bristle
      (Fujii et al., 2015)
      low salt-sensing neuron of labellar taste bristle of labellar taste bristle
      (Fujii et al., 2015)
      sugar-sensing neuron of taste bristle of labellar taste bristle
      (Fujii et al., 2015)
      prothoracic leg taste bristle chemosensory neuron of tarsal segment 4
      (Fujii et al., 2015)
      prothoracic leg taste bristle chemosensory neuron | subset of tarsal segment 5
      (Fujii et al., 2015)
      sugar-sensing neuron of taste bristle | subset of prothoracic leg taste bristle chemosensory neuron
      (Fujii et al., 2015)
      gustatory receptor neuron | subset of pseudotracheal taste peg
      (Fujii et al., 2015)
      gustatory receptor neuron | subset of labral sense organ neuron

      Comment: Expressed in a subset of gustatory receptor neurons

      (Fujii et al., 2015)
      adult subesophageal zone | restricted
      (Fujii et al., 2015)
      maxillary palp olfactory receptor neuron | subset
      (Fujii et al., 2015)
      antennal lobe glomerulus VA7l | restricted
      (Fujii et al., 2015)
      Additional Information
      Statement
      Reference

      EcollexAVP16.Gr5a drives expression in 2 (of 4) prothoracic leg tarsal taste bristles in tarsal segment 5, 6 gustatory receptor neurons associated with the taste pegs and 1-2 gustatory receptor neurons of the adult labral sense organ. All of the labellar neurons and many of the tarsal S cells send projections to the adult subesophageal zone.

      (Fujii et al., 2015)
       
      Marker for
      Reflects expression of
      Gr5a
      (Fujii et al., 2015)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2lexAop.cHa) of Ecol\lexAVP16.Gr5a cells in adult females results in a significant increase in preference for softer 100 mM sucrose-containing agarose for egg laying, compared to controls.

      (Wu et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Ecol\lexAGr5a.T:Hsim\VP16.cFa
      Ecol\lexAVP16.Gr5a
      Gr5aLexA
      (Chen et al., 2022, Masuzzo et al., 2022, Chen et al., 2019, Wu et al., 2019, Ahn et al., 2017)
      Name Synonyms
      construct a of Fujii
      Secondary FlyBase IDs
        References (9)